Invitrogen

FAS (CD95) Polyclonal Antibody

FAS (CD95) Antibody in Western Blot (WB)

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FAS (CD95) Antibody (PA5-98339) in WB

Western Blot analysis of FAS (CD95) using a FAS (CD95) Polyclonal antibody (Product # PA5-98339) at a concentration of 3.1 µg/mL. Positive WB detected in: Zebrafish heart tissue, Zebrafish liver tissue, Zebrafish brain tissue, Zebrafish eye tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.

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Product Details

PA5-98339

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Zebrafish Fas cell surface death receptor protein (185-293AA)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.13 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

AB_2812952

Target Information

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apo-1 Fas; Apo1 antigen; Fas (TNF receptor superfamily, member 6); OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646

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Entrez Gene ID: (Zebrafish) 768248