Proteintech

FASTKD2 Polyclonal Antibody

FASTKD2 Antibody in Immunocytochemistry (ICC/IF)

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FASTKD2 Antibody (17464-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using (Product # 17464-1-AP) (FASTKD2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FASTKD2 Antibody in Immunoprecipitation (IP)

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Product Details

17464-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FASTKD2 Fusion Protein Ag11314 (361-710 aa encoded by BC001544)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ILLDECSKV VLDNIHGCPL RIMINILQSC KDLQYHNLDL FKGLADYVAA TFDIWKFRKV LFILILFENL GFRPVGLMDL FMKRIVEDPE SLNMKNILSI LHTYSSLNHV YKCQNKEQFV EVMASALTGY LHTISSENLL DAVYSFCLMN YFPLAPFNQL LQKDIISELL TSDDMKNAYK LHTLDTCLKL DDTVYLRDIA LSLPQLPREL PSSHTNAKVA EVLSSLLGGE GHFSKDVHLP HNYHIDFEIR MDTNRNQVLP LSDVDTTSAT DIQRVAVLCV SRSAYCLGSS HPRGFLAMKM RHLNAMGFHV ILVNNWEMDK LEMEDAVTFL KTKIYSVEAL PVAAVNVQST Q (361-710 aa encoded by BC001544)

Target Information

This gene encodes a protein that is localized in the mitochondrial inner compartment and that may play a role in mitochondrial apoptosis. Nonsense mutations have been reported to result in cytochrome c oxidase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids.

PloS one

Nasonovs A,Garcia-Diaz M,Bogenhagen DF

Tue Oct 19 00:00:00 UTC 2021

Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.

Methods in molecular biology (Clifton, N.J.)

Choi A,Barrientos A

Mon Mar 29 00:00:00 UTC 2021

Visualization of Mitochondrial RNA Granules in Cultured Cells Using 5-Bromouridine Labeling.

Methods in molecular biology (Clifton, N.J.)

Xavier VJ,Martinou JC

Mon Mar 29 00:00:00 UTC 2021

The FASTK family proteins fine-tune mitochondrial RNA processing.

PLoS genetics

Ohkubo A,Van Haute L,Rudler DL,Stentenbach M,Steiner FA,Rackham O,Minczuk M,Filipovska A,Martinou JC

Mon Nov 01 00:00:00 UTC 2021

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.

Cell reports

Lowden C,Boulet A,Boehler NA,Seecharran S,Rios Garcia J,Lowe NJ,Liu J,Ong JLK,Wang W,Ma L,Cheng AH,Senatore A,Monks DA,Liu BH,Leary SC,Cheng HM

Tue Sep 14 00:00:00 UTC 2021

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Nature cell biology

Rey T,Zaganelli S,Cuillery E,Vartholomaiou E,Croisier M,Martinou JC,Manley S

Thu Oct 01 00:00:00 UTC 2020

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Nature cell biology

Rey T,Zaganelli S,Cuillery E,Vartholomaiou E,Croisier M,Martinou JC,Manley S

Thu Oct 01 00:00:00 UTC 2020

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Journal of cellular biochemistry

Fang R,Zhang B,Lu X,Jin X,Liu T

Sun Mar 01 00:00:00 UTC 2020

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Journal of cellular biochemistry

Fang R,Zhang B,Lu X,Jin X,Liu T

Sun Mar 01 00:00:00 UTC 2020

Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.

Human mutation

Wei X,Du M,Li D,Wen S,Xie J,Li Y,Chen A,Zhang K,Xu P,Jia M,Wen C,Zhou H,Lyu J,Yang Y,Fang H

Fri May 01 00:00:00 UTC 2020

Mitochondrial Alkbh1 localizes to mtRNA granules and its knockdown induces the mitochondrial UPR in humans and C. elegans.

Journal of cell science

Wagner A,Hofmeister O,Rolland SG,Maiser A,Aasumets K,Schmitt S,Schorpp K,Feuchtinger A,Hadian K,Schneider S,Zischka H,Leonhardt H,Conradt B,Gerhold JM,Wolf A

Tue Oct 01 00:00:00 UTC 2019

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Nucleic acids research

Boehm E,Zaganelli S,Maundrell K,Jourdain AA,Thore S,Martinou JC

Fri Jun 02 00:00:00 UTC 2017

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Nucleic acids research

Boehm E,Zaganelli S,Maundrell K,Jourdain AA,Thore S,Martinou JC

Fri Jun 02 00:00:00 UTC 2017

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

EMBO reports

Antonicka H,Choquet K,Lin ZY,Gingras AC,Kleinman CL,Shoubridge EA

Sun Jan 01 00:00:00 UTC 2017

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

EMBO reports

Antonicka H,Choquet K,Lin ZY,Gingras AC,Kleinman CL,Shoubridge EA

Sun Jan 01 00:00:00 UTC 2017

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.

The Journal of biological chemistry

Zaganelli S,Rebelo-Guiomar P,Maundrell K,Rozanska A,Pierredon S,Powell CA,Jourdain AA,Hulo N,Lightowlers RN,Chrzanowska-Lightowlers ZM,Minczuk M,Martinou JC

Fri Mar 17 00:00:00 UTC 2017

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Cell metabolism

Arroyo JD,Jourdain AA,Calvo SE,Ballarano CA,Doench JG,Root DE,Mootha VK

Tue Dec 13 00:00:00 UTC 2016

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Cell metabolism

Arroyo JD,Jourdain AA,Calvo SE,Ballarano CA,Doench JG,Root DE,Mootha VK

Tue Dec 13 00:00:00 UTC 2016

Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.

The Journal of biological chemistry

Boehm E,Zornoza M,Jourdain AA,Delmiro Magdalena A,García-Consuegra I,Torres Merino R,Orduña A,Martín MA,Martinou JC,De la Fuente MA,Simarro M

Fri Dec 09 00:00:00 UTC 2016

Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression.

The Journal of biological chemistry

Boehm E,Zornoza M,Jourdain AA,Delmiro Magdalena A,García-Consuegra I,Torres Merino R,Orduña A,Martín MA,Martinou JC,De la Fuente MA,Simarro M

Fri Dec 09 00:00:00 UTC 2016

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.

RNA (New York, N.Y.)

Popow J,Alleaume AM,Curk T,Schwarzl T,Sauer S,Hentze MW

Sun Nov 01 00:00:00 UTC 2015

A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.

Cell reports

Jourdain AA,Koppen M,Rodley CD,Maundrell K,Gueguen N,Reynier P,Guaras AM,Enriquez JA,Anderson P,Simarro M,Martinou JC

Tue Feb 24 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Cell reports

Antonicka H,Shoubridge EA

Tue Feb 17 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: FAST kinase domain-containing protein 2; FAST kinase domain-containing protein 2, mitochondrial

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Gene Aliases: 2810421I24Rik; FASTKD2; KIAA0971; RGD1307883

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UniProt ID: (Human) Q9NYY8, (Mouse) Q922E6, (Rat) Q5M7V7

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Entrez Gene ID: (Human) 22868, (Mouse) 75619, (Rat) 301463

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Function(s)

protein kinase activity rRNA binding poly(A) RNA binding

Process(es)

protein phosphorylation cellular respiration mitochondrial large ribosomal subunit assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FASTKD2 Polyclonal Antibody

FASTKD2 Antibody (17464-1-AP) in ICC/IF

Product Details

17464-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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