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Invitrogen
FBXO33 Polyclonal Antibody
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FIGURE: 1 / 1
FBXO33 Antibody (PA5-89740) in WB
Western blot analysis of FBXO33 in extracts of various cell lines. Samples were incubated with FBXO33 Polyclonal antibody (Product # PA5-89740) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-89740
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 346-555 of human FBXO33 (NP_9760461)
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805754
Product Specific Information
Immunogen sequence: VMHKSLDNMP NDEHWKALSR KSTSFRVYIM AFDIKSEDML KILKPSIPLE RIHFDSYITC VSGAIVDLIS RQYDKFLTHF ILMNDVIDTS GFPDLSDNRN EDPLVLLAWR CTKLSLLAIH GYTVWAHNLI AIARLRGSDL KVLEVTEESI DFDQGELADQ DVDPVHNLIE QVSLGLGQPW HAVMDIESLS VFTEPNRHFY REMQSFSEDI; Positive Samples: Rat brain, Rat testis
Target Information
FBXO33 is the substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO33 probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.Members of the F-box protein family, such as FBXO33, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F-box only protein 33
Entrez Gene ID: (Rat) 314157
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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