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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FBXO4 Antibodies

          Rockland

          FBXO4 Polyclonal Antibody

          View all (8) FBXO4 antibodies

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          Cite FBXO4 Polyclonal Antibody

          • Antibody Testing Data (2)
          FBXO4 Antibody in Immunocytochemistry (ICC/IF)
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          FBXO4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          FBXO4 Antibody (100-401-963) in ICC/IF

          Immunocytochemistry staining of NIH-3T3 cells with Rocklands anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          FBXO4 Antibody in Immunocytochemistry (ICC/IF)
          FBXO4 Antibody in Western Blot (WB)

          Product Details

          100-401-963

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunocytochemistry (ICC/IF)

          1:300
          -

          ELISA (ELISA)

          1:5,000-1:25,000
          -

          Immunoprecipitation (IP)

          1:500-1:3,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          85 mg/mL

          Storage buffer

          0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          This antiserum is directed against human and mouse FBX4. Reactivity against homologues from other sources is not known.

          Target Information

          This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: F-box only protein 4; F-box protein Fbx4

          View more View less

          Gene Aliases: 1700096C12Rik; AI851261; AW494535; FBX4; FBXO4

          View more View less

          UniProt ID: (Human) Q9UKT5, (Mouse) Q8CHQ0

          View more View less

          Entrez Gene ID: (Human) 26272, (Mouse) 106052

          View more View less

          Function(s)
          ubiquitin-protein transferase activity protein binding protein homodimerization activity
          Process(es)
          protein polyubiquitination telomere maintenance ubiquitin-dependent protein catabolic process protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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