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Invitrogen
FBXO4 Polyclonal Antibody
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FIGURE: 1 / 1
FBXO4 Antibody (PA5-97311) in WB
Western blot analysis of FBXO4 in extracts of various cell lines. Samples were incubated with FBXO4 Polyclonal antibody (Product # PA5-97311) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-97311
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 138-387 of human FBXO4 (NP_0363081)
Conjugate
Form
Liquid
Concentration
1.12 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809113
Product Specific Information
Immunogen sequence: DYMAVYRMCC PYTRRASKSS RPMYGAVTSF LHSLIIQNEP RFAMFGPGLE ELNTSLVLSL MSSEELCPTA GLPQRQIDGI GSGVNFQLNN QHKFNILILY STTRKERDRA REEHTSAVNK MFSRHNEGDD QQGSRYSVIP QIQKVCEVVD GFIYVANAEA HKRHEWQDEF SHIMAMTDPA FGSSGRPLLV LSCISQGDVK RMPCFYLAHE LHLNLLNHPW LVQDTEAETL TGFLNGIEWI LEEVESKRAR; Positive Samples: SH-SY5Y, Mouse spleen, Mouse kidney, Mouse liver; Cellular Location: Cytoplasm
Target Information
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F-box only protein 4; F-box protein Fbx4
Gene Aliases: 1700096C12Rik; AI851261; AW494535; FBX4; FBXO4
UniProt ID: (Human) Q9UKT5, (Mouse) Q8CHQ0
Entrez Gene ID: (Human) 26272, (Mouse) 106052
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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