Proteintech

FBXW7 Polyclonal Antibody

FBXW7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FBXW7 Antibody (28424-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

28424-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FBXW7 Fusion Protein Ag28471

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

F-Box and WD Repeat Domain Containing 7 (FBXW7) is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW7 was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pharmacological vitamin C inhibits mTOR signaling and tumor growth by degrading Rictor and inducing HMOX1 expression.

PLoS genetics

Qin S,Wang G,Chen L,Geng H,Zheng Y,Xia C,Wu S,Yao J,Deng L

Wed Feb 01 00:00:00 UTC 2023

FBXW7 loss of function promotes esophageal squamous cell carcinoma progression via elevating MAP4 and ERK phosphorylation.

Journal of experimental & clinical cancer research : CR

Pan Y,Liu J,Gao Y,Guo Y,Wang C,Liang Z,Wu M,Qian Y,Li Y,Shen J,Lu C,Ma S

Wed Mar 29 00:00:00 UTC 2023

FBXW7 loss of function promotes esophageal squamous cell carcinoma progression via elevating MAP4 and ERK phosphorylation.

Journal of experimental & clinical cancer research : CR

Pan Y,Liu J,Gao Y,Guo Y,Wang C,Liang Z,Wu M,Qian Y,Li Y,Shen J,Lu C,Ma S

Wed Mar 29 00:00:00 UTC 2023

FBXW7 loss of function promotes esophageal squamous cell carcinoma progression via elevating MAP4 and ERK phosphorylation.

Journal of experimental & clinical cancer research : CR

Pan Y,Liu J,Gao Y,Guo Y,Wang C,Liang Z,Wu M,Qian Y,Li Y,Shen J,Lu C,Ma S

Wed Mar 29 00:00:00 UTC 2023

ANXA2 as a novel substrate of FBXW7 promoting esophageal squamous cell carcinoma via ERK phosphorylation.

Biochemical and biophysical research communications

Li Z,Pan Y,Yao J,Gao Y,Qian Y,Zheng M,Ma S

Wed Mar 15 00:00:00 UTC 2023

ANXA2 as a novel substrate of FBXW7 promoting esophageal squamous cell carcinoma via ERK phosphorylation.

Biochemical and biophysical research communications

Li Z,Pan Y,Yao J,Gao Y,Qian Y,Zheng M,Ma S

Wed Mar 15 00:00:00 UTC 2023

MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7.

International immunopharmacology

Wu S,Wang Z,Zhu Y,Zhu X,Guo L,Fu Y,Zhang Q,Mou X,Liu Y

Mon May 01 00:00:00 UTC 2023

MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7.

International immunopharmacology

Wu S,Wang Z,Zhu Y,Zhu X,Guo L,Fu Y,Zhang Q,Mou X,Liu Y

Mon May 01 00:00:00 UTC 2023

MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7.

International immunopharmacology

Wu S,Wang Z,Zhu Y,Zhu X,Guo L,Fu Y,Zhang Q,Mou X,Liu Y

Mon May 01 00:00:00 UTC 2023

MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7.

International immunopharmacology

Wu S,Wang Z,Zhu Y,Zhu X,Guo L,Fu Y,Zhang Q,Mou X,Liu Y

Mon May 01 00:00:00 UTC 2023

PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop.

Cancer communications (London, England)

Zhao X,Shu D,Sun W,Si S,Ran W,Guo B,Cui L

Sat Oct 01 00:00:00 UTC 2022

EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway.

Frontiers in immunology

Wu J,Luo FL,Xie Y,Xiong H,Gao Y,Liu G,Zhang XL

Thu Nov 16 00:00:00 UTC 2023

EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway.

Frontiers in immunology

Wu J,Luo FL,Xie Y,Xiong H,Gao Y,Liu G,Zhang XL

Thu Nov 16 00:00:00 UTC 2023

EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway.

Frontiers in immunology

Wu J,Luo FL,Xie Y,Xiong H,Gao Y,Liu G,Zhang XL

Thu Nov 16 00:00:00 UTC 2023

The lncRNA BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in gastric cancer peritoneal metastasis by regulating VDAC3 ubiquitination.

International journal of biological sciences

Huang G,Xiang Z,Wu H,He Q,Dou R,Lin Z,Yang C,Huang S,Song J,Di Z,Wang S,Xiong B

Fri Apr 15 00:00:00 UTC 2022

Notch activation suppresses endothelial cell migration and sprouting via miR-223-3p targeting Fbxw7.

In vitro cellular & developmental biology. Animal

Wang R,Yang Z,Liang L,Feng X,Che B,Zhang X,Zheng Q,Yan X,Han H

Tue Feb 01 00:00:00 UTC 2022

A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.

Journal of experimental & clinical cancer research : CR

Liu W,Ren D,Xiong W,Jin X,Zhu L

Wed Jan 26 00:00:00 UTC 2022

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Cell reports

Li Q,Yao H,Wang Y,Wu Y,Thorne RF,Zhu Y,Wu M,Liu L

Tue Nov 22 00:00:00 UTC 2022

MicroRNA‑223‑induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways.

Molecular medicine reports

Liu Z,Ma T,Duan J,Liu X,Liu L

Mon Feb 01 00:00:00 UTC 2021

Dihydrocapsaicin Inhibits Cell Proliferation and Metastasis in Melanoma via Down-regulating β-Catenin Pathway.

Frontiers in oncology

Shi S,Li C,Zhang Y,Deng C,Liu W,Du J,Li Q,Ji Y,Guo L,Liu L,Hu H,Liu Y,Cui H

Sat Nov 11 00:00:00 UTC 2023

Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.

Cell biology international

Wang CH,Lu TJ,Wang LK,Wu CC,Chen ML,Kuo CY,Shyu RY,Tsai FM

Mon Nov 01 00:00:00 UTC 2021

Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.

Frontiers in cell and developmental biology

Zhu T,Liu B,Wu D,Xu G,Fan Y

Tue Dec 07 00:00:00 UTC 2021

Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.

Frontiers in cell and developmental biology

Zhu T,Liu B,Wu D,Xu G,Fan Y

Tue Dec 07 00:00:00 UTC 2021

Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression.

Theranostics

Yu C,Xiong C,Tang J,Hou X,Liu N,Bayliss G,Zhuang S

Tue Jul 27 00:00:00 UTC 2021

Aberrant FBXW7-mediated ubiquitination and degradation of ZMYND8 enhances tumor progression and stemness in bladder cancer.

Experimental cell research

Qiu F,Jin Y,Pu J,Huang Y,Hou J,Zhao X,Lu Y

Fri Oct 15 00:00:00 UTC 2021

Aberrant FBXW7-mediated ubiquitination and degradation of ZMYND8 enhances tumor progression and stemness in bladder cancer.

Experimental cell research

Qiu F,Jin Y,Pu J,Huang Y,Hou J,Zhao X,Lu Y

Fri Oct 15 00:00:00 UTC 2021

Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells.

Molecular cancer

Yeh CH,Bellon M,Wang F,Zhang H,Fu L,Nicot C

Wed Sep 09 00:00:00 UTC 2020

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Cellular signalling

Cooper LM,Hanson A,Kavanagh JA,Waddell DS

Mon Jun 01 00:00:00 UTC 2020

The 18S rRNA m⁶ A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.

EMBO reports

Xing M,Liu Q,Mao C,Zeng H,Zhang X,Zhao S,Chen L,Liu M,Shen B,Guo X,Ma H,Chen H,Zhang J

Mon Oct 05 00:00:00 UTC 2020

Lycorine hydrochloride inhibits cell proliferation and induces apoptosis through promoting FBXW7-MCL1 axis in gastric cancer.

Journal of experimental & clinical cancer research : CR

Li C,Deng C,Pan G,Wang X,Zhang K,Dong Z,Zhao G,Tan M,Hu X,Shi S,Du J,Ji H,Wang X,Yang L,Cui H

Fri Oct 30 00:00:00 UTC 2020

Lycorine hydrochloride inhibits cell proliferation and induces apoptosis through promoting FBXW7-MCL1 axis in gastric cancer.

Journal of experimental & clinical cancer research : CR

Li C,Deng C,Pan G,Wang X,Zhang K,Dong Z,Zhao G,Tan M,Hu X,Shi S,Du J,Ji H,Wang X,Yang L,Cui H

Fri Oct 30 00:00:00 UTC 2020

Long non-coding RNA SSTR5-AS1 facilitates gemcitabine resistance via stabilizing NONO in gallbladder carcinoma.

Biochemical and biophysical research communications

Xue Z,Yang B,Xu Q,Zhu X,Qin G

Wed Feb 19 00:00:00 UTC 2020

TRIP13 promotes the cell proliferation, migration and invasion of glioblastoma through the FBXW7/c-MYC axis.

British journal of cancer

Zhang G,Zhu Q,Fu G,Hou J,Hu X,Cao J,Peng W,Wang X,Chen F,Cui H

Sun Dec 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: archipelago; Archipelago homolog; DKFZp686F23254; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box and WD-40 domain protein 7, archipelago homolog; F-box and WD-40 domain-containing protein 7; f-box only protein 30; F-box protein FBW7; F-box protein FBX30; F-box protein Fbxw6; F-box protein SEL-10; F-box-WD40 repeat protein 6; F-box/WD repeat-containing protein 7; FLJ11071; FLJ16457; hAgo; hCdc4; homolog of C elegans sel-10; SEL-10

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Gene Aliases: 1110001A17Rik; AGO; CDC4; FBW6; FBW7; Fbwd6; FBX30; FBXO30; FBXW6; FBXW7; hAgo; hCdc4; SEL-10; SEL10

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UniProt ID: (Human) Q969H0, (Mouse) Q8VBV4

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Entrez Gene ID: (Human) 55294, (Mouse) 50754

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Function(s)

ubiquitin-protein transferase activity protein binding cyclin binding protein binding, bridging ubiquitin protein ligase binding identical protein binding phosphothreonine binding ubiquitin-protein transferase activator activity transcription factor activity, sequence-specific DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FBXW7 Polyclonal Antibody

FBXW7 Antibody (28424-1-AP) in IHC (P)

Product Details

28424-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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