Invitrogen

FDC Monoclonal Antibody (CNA.42), Biotin, eBioscience™

FDC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 1

FDC Antibody (13-9968-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil using no primary antibody (left) or 30 µg/mL Anti-Human FDC Biotin (right) followed by streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.

Product Image Gallery

Product Details

13-9968-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (Paraffin) (IHC (P))

30 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

30 µg/mL

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

CNA.42

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572847

Product Specific Information

Description: The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells).

This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.

Applications Reported: This CNA.42 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections.

Applications Tested: This CAN.42 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue using high pH antigen retrieval. This antibody can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more