Invitrogen

FDC Monoclonal Antibody (CNA.42), eBioscience™

FDC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 1

FDC Antibody (14-9968-95) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil using 1 µg/mL Mouse IgM Isotype Control (left) or 1 µg/mL Anti-Human FDC Purified (right) followed by anti-Mouse IgM Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.

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Product Details

14-9968-95

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/Test

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

CNA.42

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2865546

Product Specific Information

Description: The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells).

This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.

Applications Reported: This CNA.42 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemistry on frozen and formalin-fixed paraffin embedded tissue sections.

Applications Tested: This CNA.42 antibody has been tested by flow cytometric of a human cell line and western blotting. This can be used at less than or equal to 0.5 µg per million cells in a 100 µL total staining volume or 2 µg/mL (for western blotting). The antibody has also been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil using high pH antigen antigen retrieval. For IHC applications, the antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of lymphoid tissue SPARC in the pathogenesis and response to treatment of multiple myeloma.

Frontiers in oncology

Aly NAR,Rizk S,Aboul Enein A,El Desoukey N,Zawam H,Ahmed M,El Shikh ME,Pitzalis C

14-9968-82 was used in Immunohistochemistry-immunofluorescence to investigate the role of secreted protein acidic and rich in cysteine (SPARC) in the pathogenesis of multiple myeloma.

Wed Mar 22 00:00:00 UTC 2023

Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis.

Frontiers in medicine

El Shikh MEM,El Sayed R,Aly NAR,Prediletto E,Hands R,Fossati-Jimack L,Bombardieri M,Lewis MJ,Pitzalis C

14-9968-82 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate that CNA.42+ FDCs distinctively express the pericyte/fibroblast-associated markers PDGFR-ß, NG2, and Thy-1 in the synovial perivascular space but not in established follicles.

Wed Mar 22 00:00:00 UTC 2023

Tertiary Lymphoid Structures in Rheumatoid Arthritis: NF-κB-Inducing Kinase-Positive Endothelial Cells as Central Players.

The American journal of pathology

Noort AR,van Zoest KP,van Baarsen LG,Maracle CX,Helder B,Papazian N,Romera-Hernandez M,Tak PP,Cupedo T,Tas SW

14-9968 was used in Immunohistochemistry on frozen tissues to investigate the relation between NIK(+) endothelial cells, precursor follicular dendritic cells, and group 3 innate lymphoid cells.

Wed Jul 01 00:00:00 UTC 2015

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