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Invitrogen
FEN1 Polyclonal Antibody
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FIGURE: 1 / 8
FEN1 Antibody (PA5-27518) in ICC/IF
Immunofluorescence analysis of FEN1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FEN1 Polyclonal Antibody (Product # PA5-27518) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a ... View More
Product Details
PA5-27518
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 1% BSA, 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544994
Product Specific Information
Recommended positive controls: HepG2, NIH-3T3, JC, BCL-1, Raw264.7, PC-12.
Predicted reactivity: Mouse (96%), Rat (95%), Chicken (81%), Sheep (94%), Rhesus Monkey (98%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNase IV; FEN-1; FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}; FEN1; fen1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap endonuclease 1; flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap structure-specific endonuclease 1; flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; hFEN-1; Maturation factor 1; maturation factor-1; MF1
Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2
UniProt ID: (Human) P39748, (Rat) Q5XIP6
Entrez Gene ID: (Human) 2237, (Mouse) 14156, (Rat) 84490
magnesium ion binding
DNA binding
damaged DNA binding
double-stranded DNA binding
endonuclease activity
RNA-DNA hybrid ribonuclease activity
exonuclease activity
protein binding
double-stranded DNA exodeoxyribonuclease activity
5'-3' exonuclease activity
5'-flap endonuclease activity
catalytic activity
nuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
manganese ion binding
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
metal ion binding
flap endonuclease activity
DNA metabolic process
DNA replication
DNA repair
cellular response to DNA damage stimulus
memory
DNA replication, removal of RNA primer
nucleic acid phosphodiester bond hydrolysis
RNA phosphodiester bond hydrolysis, endonucleolytic
DNA catabolic process, endonucleolytic
base-excision repair
telomere maintenance via recombination
double-strand break repair via homologous recombination
double-strand break repair
UV protection
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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