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FGF16 Antibody (500-P160GBT-25UG) in WB
Western Blot: To detect hFGF-16 by Western Blot analysis FGF16 Polyclonal Antibody, Biotin (Product # 500-P160GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P160GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human FGF-16
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929553
Product Specific Information
AA Sequence of recombinant protein: AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human FGF-16. Anti-Human FGF-16-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human FGF-16 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-16 (500-P160G) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human FGF-16
Western Blot: To detect hFGF-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P160GBT-1MG will be provided as 2 x 500 µg
Target Information
FGF-16 is a heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-16 signals through FGFR 2c and 3c. FGF-16 plays a role in the development of the central nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-16; Fibroblast growth factor; Fibroblast growth factor 16; metacarpal 4-5 fusion
Gene Aliases: FGF-16; FGF16; MF4
UniProt ID: (Human) O43320
Entrez Gene ID: (Human) 8823
MAPK cascade
regulation of endothelial cell proliferation
signal transduction
cell-cell signaling
metabolic process
fibroblast growth factor receptor signaling pathway
response to temperature stimulus
organ morphogenesis
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
phosphatidylinositol-3-phosphate biosynthetic process
regulation of MAP kinase activity
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of brown fat cell proliferation
regulation of sprouting angiogenesis
positive regulation of endothelial cell chemotaxis to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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