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Product Details
710148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
Recombinant protein corresponding to amino acids 163-285 of human FGF2 (basic)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532590
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; FGF-2; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b; prostatropin
Gene Aliases: BFGF; FGF-2; FGF2; FGFB; HBGF-2
UniProt ID: (Human) P09038
Entrez Gene ID: (Human) 2247
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
cytokine activity
protein binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
ligand-dependent nuclear receptor transcription coactivator activity
chemoattractant activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
activation of MAPK activity
branching involved in ureteric bud morphogenesis
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
phosphatidylinositol biosynthetic process
chemotaxis
signal transduction
Ras protein signal transduction
nervous system development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
organ morphogenesis
negative regulation of fibroblast migration
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
growth factor dependent regulation of skeletal muscle satellite cell proliferation
peptidyl-tyrosine phosphorylation
extracellular matrix organization
hyaluronan catabolic process
inositol phosphate biosynthetic process
somatic stem cell population maintenance
phosphatidylinositol-3-phosphate biosynthetic process
wound healing
positive regulation of cell fate specification
positive regulation of MAP kinase activity
positive regulation of blood vessel endothelial cell migration
negative regulation of blood vessel endothelial cell migration
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
embryonic morphogenesis
positive chemotaxis
release of sequestered calcium ion into cytosol
positive regulation of cell division
positive regulation of cardiac muscle cell proliferation
negative regulation of cell death
chondroblast differentiation
negative regulation of wound healing
positive regulation of ERK1 and ERK2 cascade
positive regulation of sprouting angiogenesis
regulation of endothelial cell chemotaxis to fibroblast growth factor
positive regulation of endothelial cell chemotaxis to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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