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Invitrogen
FGF4 Recombinant Rabbit Monoclonal Antibody (2H7L18)
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FGF4 Antibody (703064) in ICC/IF
For immunofluorescence analysis, induced pluripotent stem (iPS) cells were fixed and permeabilized for detection of endogenous FGF4 using Anti-FGF4 Recombinant Rabbit Monoclonal Antibody (Product # 703064, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel a-c) clearly demonstrates cytoplasmic localization of FGF4 in iPS cells. Panel (d-f) sho... View More
Product Details
703064
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H7L18
Immunogen
Protein corresponding to human FGF4 [aa79-aa206]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784557
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3
Gene Aliases: FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; KS3
UniProt ID: (Human) P08620
Entrez Gene ID: (Human) 2249
MAPK cascade
cartilage condensation
signal transduction
cell-cell signaling
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
mesenchymal cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
stem cell population maintenance
embryonic hindlimb morphogenesis
phosphatidylinositol-3-phosphate biosynthetic process
odontogenesis of dentin-containing tooth
negative regulation of apoptotic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of cell division
cranial suture morphogenesis
apoptotic process involved in morphogenesis
chondroblast differentiation
positive regulation of ERK1 and ERK2 cascade
regulation of endothelial cell chemotaxis to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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