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Invitrogen
FHL3 Polyclonal Antibody
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FIGURE: 1 / 1
FHL3 Antibody (PA5-95379) in WB
Western blot analysis of FHL3 in, Lane 1: human K562 whole cell lysates, Lane 2: rat heart tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with FHL3 Polyclonal Antibody (P... View More
Product Details
PA5-95379
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FHL3 (41-84aa AECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807182
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, rat heart tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FHL-3; Four and a half LIM domains protein 3; LIM-only protein FHL3; MGC23614; MGC8696; Skeletal muscle LIM-protein 2; SLIM-2
Gene Aliases: FHL3; SLIM2
UniProt ID: (Human) Q13643
Entrez Gene ID: (Human) 2275, (Rat) 313582
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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