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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FITC Antibodies

          Invitrogen

          FITC Polyclonal Antibody

          11 Published Figures
          26 References
          View all (41) FITC antibodies

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          Datasheet
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          Datasheet
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          Cite FITC Polyclonal Antibody

          • Published Figures (11)
          FITC Antibody in Immunocytochemistry (ICC/IF)
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          FITC Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          FITC Antibody (71-1900) in ICC/IF

          Nature communications 2018 - Fig. 2 GLP-1R agonist trafficking in MIN6B1-SNAP-GLP-1R cells. a Schematic for GLP-1R internalization and recycling measurements by FACS after labeling with cleavable SNAP-Surface probe. b Agonist-induced GLP-1R internalization in MIN6B1-SNAP-GLP-1R cells, n = 3, two-way randomized block ANOVA with Dunnett's test vs. exendin-4. c GLP-1R recycling in MIN6B1-SNAP-GLP-1R cells 30 min after an initial 15 min ag... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          FITC Antibody in Immunocytochemistry (ICC/IF)
          FITC Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          FITC Antibody in Immunohistochemistry (IHC)
          FITC Antibody in Immunohistochemistry (IHC)
          FITC Antibody in Immunohistochemistry (IHC)
          FITC Antibody in Western Blot, Immunoprecipitation (WB, IP)
          FITC Antibody in Western Blot (WB)
          FITC Antibody in Western Blot (WB)
          FITC Antibody in Western Blot (WB)
          FITC Antibody in Western Blot (WB)
          FITC Antibody in Immunomicroscopy (IM)

          Product Details

          71-1900

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-1 µg
          View 4 publications 4 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 9 publications 9 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          ELISA (ELISA)

          0.5-1.0 µg/mL
          -

          Immunoprecipitation (IP)

          Assay-dependent
          View 2 publications 2 publications

          Immunomicroscopy (IM)

          -
          View 1 publication 1 publication

          In Situ Hybridization (ISH) (ISH)

          -
          View 1 publication 1 publication

          Affinity Purification (AP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical, Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          FITC

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533978

          Product Specific Information

          This antibody was purified from serum of rabbit hyper-immunized with FITC conjugated to KLH. The antibody was purified by antigen-specific matrix. The antibody reacts with FITC but not the carrier protein. Useful as a secondary antibody in a wide range of immunoassays including: ELISA, tissue and cell staining, immunoprecipitation, and western blotting. The protein concentration is determined based on an E (1%) = 14 at 280 nm.

          Target Information

          FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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