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Invitrogen
FKBP15 Polyclonal Antibody
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FIGURE: 1 / 10
FKBP15 Antibody (PA5-144010) in ICC/IF
Immunocytochemistry analysis of FKBP15 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in FKBP15 Polyclonal antibody (Product # PA5-144010) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriat... View More
Product Details
PA5-144010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human FKBP135/FKBP15 recombinant protein (Position: A19-D1210).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075224
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hacat whole cell, human Raji whole cell, mouse lung tissue. IHC: human spleen tissue, human tonsil tissue, mouse brain tissue, rat brain tissue, human breast tissue, human lymphoma tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 133 kDa FK506-binding protein; 133 kDa FKBP; FK506 binding protein 15; FK506 binding protein 15, 133kDa; FK506-binding protein 133; FK506-binding protein 133kDa; FK506-binding protein 15; FKBP-133; FKBP-15; protein phosphatase 1, regulatory subunit 76; WAFL; WASP and FKBP-like; WASP and FKBP-like protein; WASP- and FKBP-like protein
Gene Aliases: BB131447; C430014M02Rik; FKBO133; FKBP-133; FKBP-15; FKBP133; FKBP15; KIAA0674; mKIAA0674; PPP1R76; RGD1304816
UniProt ID: (Human) Q5T1M5, (Mouse) Q6P9Q6
Entrez Gene ID: (Human) 23307, (Mouse) 338355, (Rat) 362528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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