Invitrogen

FKBP5 Polyclonal Antibody

9 Published Figures

19 References

View all (25) FKBP5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (9)

FIGURE: 1 / 12

FKBP5 Antibody (PA1-020) in WB

Western blot was performed using Anti-FKBP5 Polyclonal Antibody (Product # PA1-020) and a 51kDa band corresponding to FKBP5 was observed across all the tested cell lines and tissues. Whole cell extracts (30 µg lysate) of PC-12 (Lane 1), L6 (Lane 2), Rat Liver (Lane 3), Rat Brain (Lane 4), Rat Testis (Lane 5), Rat Kidney (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More

FKBP5 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-020

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2103140

Product Specific Information

PA1-020 detects FK506 binding protein 51 kDa (FKBP51) in canine, hamster, human, mouse and rat tissues.

PA1-020 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~51 kDa protein representing FKBP51 in HeLa cell lysate. By Western blot PA1-020 also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

The PA1-020 immunogen is a synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51. There is a single amino acid substitution (K396 to R396) in this domain between human and mouse FKBP51 protein. PA1-020 immunizing peptide (Cat. # PEP-078) is available for use in neutralization and control experiments.

Target Information

FKBP5 is a member of the immunophilin protein family. This family plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP5 is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. The gene that encodes FKBP5 has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel Oncogenic Transcription Factor Cooperation in RB-Deficient Cancer.

Cancer research

Mandigo AC,Shafi AA,McCann JJ,Yuan W,Laufer TS,Bogdan D,Gallagher L,Dylgjeri E,Semenova G,Vasilevskaya IA,Schiewer MJ,McNair CM,de Bono JS,Knudsen KE

PA1-020 was used in Western Blot to reveal that E2F1 binding is repositioned after retinoblastoma tumor suppressor loss to cooperate with androgen receptor and drive resultant gene expression networks that induce protumorigenic phenotypes.

Sat Jan 15 00:00:00 UTC 2022

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-020 was used in Western Blotting to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 UTC 2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-020 was used in Western Blotting to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 UTC 2020

Nucleocytoplasmic shuttling of the glucocorticoid receptor is influenced by tetratricopeptide repeat-containing proteins.

Journal of cell science

Mazaira GI,Echeverria PC,Galigniana MD

PA1-020 was used in Western Blotting to show that nuclear import of GR was impaired, whereas GR nuclear export was enhanced.

Tue Jun 16 00:00:00 UTC 2020

Heme Oxygenase 1 Impairs Glucocorticoid Receptor Activity in Prostate Cancer.

International journal of molecular sciences

Leonardi DB,Anselmino N,Brandani JN,Jaworski FM,Páez AV,Mazaira G,Meiss RP,Nuñez M,Nemirovsky SI,Giudice J,Galigniana M,Pecci A,Gueron G,Vazquez E,Cotignola J

PA1-020 was used in Western Blotting to identify the molecular processes governed by the interaction between haem oxygenase 1 and glucocorticoid receptor.

Tue Feb 26 00:00:00 UTC 2019

In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network.

FEBS open bio

Bernadotte A,Kumar R,Winblad B,Pavlov PF

PA1-020 was used in Western Blot to identify and characterise the human dicarboxylate clamp tetratricopeptide repeat protein interaction network.

Thu Nov 01 00:00:00 UTC 2018

Western High-Fat Diet Consumption during Adolescence Increases Susceptibility to Traumatic Stress while Selectively Disrupting Hippocampal and Ventricular Volumes.

eNeuro

Kalyan-Masih P,Vega-Torres JD,Miles C,Haddad E,Rainsbury S,Baghchechi M,Obenaus A,Figueroa JD

PA1020 was used in western blot to test if a Western-like high-fat diet predisposes rats to post-traumatic stress responsivity

Thu Oct 26 00:00:00 UTC 2017

Pharmacological stimulation of hypoxia inducible factor-1α facilitates the corticosterone response to a mild acute stressor.

Neuroscience letters

Harrell CS,Rowson SA,Neigh GN

PA1-020 was used in western blot to determine if HIF-1 links glucocorticoids and oxidative stress

Thu Jul 23 00:00:00 UTC 2015

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

PA1-020 was used in immunocytochemistry and western blot to investigate the alterations hsp90-binding immunophilins distribution during neuronal growth and differentiation

Mon Nov 01 00:00:00 UTC 2010

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

PA1-020 was used in immunocytochemistry and western blot to investigate the alterations hsp90-binding immunophilins distribution during neuronal growth and differentiation

Mon Nov 01 00:00:00 UTC 2010

PHLPP1 splice variants differentially regulate AKT and PKCα signaling in hippocampal neurons: characterization of PHLPP proteins in the adult hippocampus.

Journal of neurochemistry

Jackson TC,Verrier JD,Semple-Rowland S,Kumar A,Foster TC

PA1-020 was used in western blot to investigate the functions of different PHLPP splice variants in adult hippocampus

Mon Nov 01 00:00:00 UTC 2010

The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events.

Molecular and cellular biology

Galigniana MD,Erlejman AG,Monte M,Gomez-Sanchez C,Piwien-Pilipuk G

PA1-020 was used in western blot to investigate the role of the heat shock protein 90-FKBP52 complex in the nucleus

Mon Mar 01 00:00:00 UTC 2010

Comparative analysis of calcineurin inhibition by complexes of immunosuppressive drugs with human FK506 binding proteins.

Biochemistry

Weiwad M,Edlich F,Kilka S,Erdmann F,Jarczowski F,Dorn M,Moutty MC,Fischer G

PA1-020 was used in western blot to study the inhibition of calcineurin by immunosuppressive drug-FK506 binding protein complexes

Tue Dec 26 00:00:00 UTC 2006

Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kerns RT,Ravindranathan A,Hassan S,Cage MP,York T,Sikela JM,Williams RW,Miles MF

Published figure using FKBP5 polyclonal antibody (Product # PA1-020) in Western Blot

Wed Mar 02 00:00:00 UTC 2005

Up-regulation of glucocorticoid-regulated genes in a mouse model of Rett syndrome.

Human molecular genetics

Nuber UA,Kriaucionis S,Roloff TC,Guy J,Selfridge J,Steinhoff C,Schulz R,Lipkowitz B,Ropers HH,Holmes MC,Bird A

PA1-020 was used in western blot to study the expression of glucocorticoid-regulated genes in Rett syndrome mouse model.

Mon Aug 01 00:00:00 UTC 2005

Differential control of glucocorticoid receptor hormone-binding function by tetratricopeptide repeat (TPR) proteins and the immunosuppressive ligand FK506.

Biochemistry

Davies TH,Ning YM,Sánchez ER

PA1-020 was used in western blot to investigate the regulatory effects of FK506 and TPR proteins on glucocorticoid receptor hormone-binding function

Tue Feb 15 00:00:00 UTC 2005

Progesterone receptor deficient in chromatin binding has an altered cellular state.

The Journal of biological chemistry

Botos J,Xian W,Smith DF,Smith CL

PA1-020 was used in western blot to analyze the altered cellular processing by comparing the composition and distribution of tPR and sPR complexes

Fri Apr 09 00:00:00 UTC 2004

Association of immunophilins with mammalian TRPC channels.

The Journal of biological chemistry

Sinkins WG,Goel M,Estacion M,Schilling WP

PA1-020 was used in western blot to study the role of immunophilins in TRPC channel activation following receptor stimulation.

Fri Aug 13 00:00:00 UTC 2004

A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins.

The Journal of biological chemistry

Davies TH,Ning YM,Sánchez ER

PA1-020 was used in western blot to investigate the activation mechanism for glucocorticoid receptors.

Fri Feb 15 00:00:00 UTC 2002

Tissue distribution and abundance of human FKBP51, and FK506-binding protein that can mediate calcineurin inhibition.

Biochemical and biophysical research communications

Baughman G,Wiederrecht GJ,Chang F,Martin MM,Bourgeois S

PA1-020 was used in western blot to study the expression of FK506-binding protein FKBP51 in tissues

Mon Mar 17 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; EC 5.2.1.8; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP-5; FKBP-51; FKBP54; HSP90-binding immunophilin; p54; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase FKBP5; Rotamase; T-cell FK506-binding protein

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Gene Aliases: AIG6; D17Ertd592e; Dit1; FKBP-5; FKBP5; FKBP51; FKBP54; P54; PPIase; Ptg-10

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UniProt ID: (Human) Q13451, (Mouse) Q64378

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Entrez Gene ID: (Human) 2289, (Dog) 481759, (Mouse) 14229, (Rat) 361810, (Chinese hamster) 100760904

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Function(s)

peptidyl-prolyl cis-trans isomerase activity protein binding FK506 binding heat shock protein binding isomerase activity molecular_function

Process(es)

protein peptidyl-prolyl isomerization protein folding chaperone-mediated protein folding biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FKBP5 Polyclonal Antibody

FKBP5 Antibody (PA1-020) in WB

Product Details

PA1-020

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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