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FLI1 Antibody (PA5-96374) in WB
Western Blot was performed using Anti-FLI1 Polyclonal Antibody (Product # PA5-96374) and a 50 kDa band corresponding to Friend leukemia integration 1 transcription factor was observed across all the cell lines tested except for the Raji cell line where a 55 kDa band was also observed. Nuclear enriched extracts (30 µg lysate) of MCF 10A (Lane 1), SK-BR-3 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), HEL 92.1.7 (Lane 5), Raji (Lane 6), K-562 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved pr... View More
Product Details
PA5-96374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_0020082)
Conjugate
Form
Liquid
Concentration
0.39 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808176
Product Specific Information
Immunogen sequence: MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ PVRVNVKREY DHMNGSRESP VDCSVSKCSK LVGGGESNPM; Positive Samples: THP-1, Jurkat, HepG2, Mouse heart; Cellular Location: Nucleus
Target Information
ETS-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU. 1 and PEA3. Members of the ETS gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ewing sarcoma breakpoint region 2; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; Retroviral integration site protein Fli-1; Transcription factor ERGB
Gene Aliases: EWSR2; Fli-1; FLI1; SIC-1; Sic1
UniProt ID: (Human) Q01543, (Mouse) P26323
Entrez Gene ID: (Human) 2313, (Mouse) 14247
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
hemostasis
blood circulation
organ morphogenesis
cell differentiation
megakaryocyte development
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular process
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