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Invitrogen
FLT3 (CD135) Polyclonal Antibody
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FLT3 (CD135) Antibody (PA5-79270) in IHC (P)
Immunohistochemistry analysis of FLT3 (CD135) on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with FLT3 (CD135) polyclonal antibody (Product# PA5-79270) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 mi... View More
Product Details
PA5-79270
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746386
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse brain tissue. IHC: mouse small intestine tissue, rat kidney tissue, rat liver tissue, rat small intestine tissue, mouse kidney tissue.
Target Information
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2
Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl
UniProt ID: (Mouse) Q00342
Entrez Gene ID: (Rat) 140635, (Mouse) 14255
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
cytokine receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
ubiquitin protein ligase binding
protein complex binding
glucocorticoid receptor binding
phosphatidylinositol 3-kinase binding
leukocyte homeostasis
positive regulation of protein phosphorylation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
pro-B cell differentiation
pro-T cell differentiation
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
negative regulation of cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
hemopoiesis
lymphocyte differentiation
cell differentiation
B cell differentiation
common myeloid progenitor cell proliferation
negative regulation of B cell differentiation
lymphocyte proliferation
protein autophosphorylation
cellular response to cytokine stimulus
dendritic cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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