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FIGURE: 1 / 1
FMRP Antibody (711441) in ICC/IF
For immunofluorescence analysis, SHSY-5Y cells were fixed and permeabilized for detection of endogenous FMR1 using Anti- FMR1 Recombinant Rabbit Superclonal™ Antibody (Product # 711441, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of FMR1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36... View More
Product Details
711441
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
5HCLC
Immunogen
Protein corresponding to Human FMR1 (216-425)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633176
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Pig and Sheep
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
FMR1 binds RNA and is associated with polysomes. The protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure. Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FMRP; fragile X mental retardation protein 1; Fragile X messenger ribonucleoprotein; Fragile X messenger ribonucleoprotein 1; MGC87458; Protein FMR-1
Gene Aliases: FMR1; FMRP; FRAXA; POF; POF1
UniProt ID: (Human) Q06787
Entrez Gene ID: (Human) 2332
G-quadruplex RNA binding
chromatin binding
RNA binding
mRNA binding
mRNA 3'-UTR binding
protein binding
microtubule binding
poly(U) RNA binding
translation repressor activity
translation initiation factor binding
RNA strand annealing activity
poly(G) binding
methylated histone binding
siRNA binding
miRNA binding
RNA stem-loop binding
identical protein binding
protein homodimerization activity
ribosome binding
ion channel binding
poly(A) RNA binding
dynein binding
protein heterodimerization activity
mRNA 5'-UTR binding
sequence-specific mRNA binding
regulation of alternative mRNA splicing, via spliceosome
positive regulation of receptor internalization
mRNA processing
cellular response to DNA damage stimulus
glutamate receptor signaling pathway
RNA splicing
negative regulation of translation
gene silencing by RNA
positive regulation of histone phosphorylation
cellular response to UV
regulation of mRNA stability
modulation by host of viral RNA genome replication
positive regulation of translation
negative regulation of translational initiation
regulation of neurotransmitter secretion
mRNA transport
regulation of filopodium assembly
positive regulation of filopodium assembly
regulation of gene silencing by miRNA
regulation of dendritic spine development
positive regulation of dendritic spine development
cellular response to hydroxyurea
cellular response to virus
regulation of neuronal action potential
negative regulation of long term synaptic depression
positive regulation of intracellular transport of viral material
negative regulation of voltage-gated calcium channel activity
positive regulation of proteasomal protein catabolic process
positive regulation of mRNA binding
negative regulation of synaptic vesicle exocytosis
positive regulation of gene silencing by miRNA
negative regulation of cytoplasmic translation
positive regulation of response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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