Invitrogen

FOXA3 Polyclonal Antibody

2 Published Figures

View all (5) FOXA3 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (3)
Published Figures (2)

FOXA3 Antibody in Immunohistochemistry (IHC)

FOXA3 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 5

FOXA3 Antibody (PA5-106980) in IHC

Immunohistochemistry of FOXA3 in human lung using a FOXA3 Polyclonal Antibody (Product # PA5-106980).

FOXA3 Antibody in Immunohistochemistry (IHC)

FOXA3 Antibody in Western Blot (WB)

FOXA3 Antibody in Western Blot (WB)

FOXA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FOXA3 Antibody in Western Blot (WB)

Product Image Gallery

FOXA3 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of FOXA3 in human lung using a FOXA3 Polyclonal Antibody (Product # PA5-106980).

FOXA3 Antibody in Western Blot (WB) — Western Blot analysis of FOXA3 in mouse skeletal muscle using a FOXA3 Polyclonal Antibody (Product # PA5-106980) diluted to 0.2-1 µg/mL.

FOXA3 Antibody in Western Blot (WB) — Western Blot analysis of FOXA3 in human Jurkat cells using a FOXA3 Polyclonal Antibody (Product # PA5-106980) diluted to 0.2-1 µg/mL.

FOXA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Knockdown of HOXC10 suppresses ESCC tumorigenesis in vivo . (A) The murine tumor images in the shCtrl and shHOXC10-1 groups (n = 5). (B) Mouse tumor size curves and (C) weight in the two groups (n = 5). (D) HE staining was conducted to evaluate the morphological changes in mouse tumor tissues. (E) IHC assays were used to measure the protein expression of Ki-67, HOXC10, and FOXA3 in mouse tumors. All experiments were triplicated. Scale bar = 50 mum **P < 0.01 , ***P < 0.001 . Fig. 6

FOXA3 Antibody in Western Blot (WB) — Fig. 5 HOXC10 enhances ESCC cell malignancy by upregulating FOXA3. (A) qRT-PCR and (B) Western blot measured FOXA3 overexpression efficacy in ESCC cells. (C) ESCC cell after silencing HOXC10 and/or overexpressing FOXA3 was evaluated using CCK-8 assays. (D) Transwell and (E) wound scratch assays were used to assess invasiveness and migrating capacity of ESCC cells. (F) Flow cytometry was used toexamine apoptosis in ESCC cells after the indicated transfections. All experiments were triplicated. Scale bar = 50 mum **P < 0.01 , ***P < 0.001 ; ###P < 0.001 . Fig. 5

Product Details

PA5-106980

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human FOXA3 (aa 201-250)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.4, with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2817696

Product Specific Information

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 100%; Zebrafish: 79%

Immunogen sequence: FENGCYLRRQ KRFKLEEKVK KGGSGAATTT RNGTGSAAST TTPAATVTSP

Target Information

FOXA3 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Western Blot to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Western Blot to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Western Blot to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Western Blot to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Western Blot to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: Fork head-related protein FKH H3; Forkhead box protein A3; hepatocyte nuclear factor 3 gamma (winged helix transcription factor); hepatocyte nuclear factor 3, gamma; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; TCF-3G; Transcription factor 3G

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Gene Aliases: FKHH3; FOXA3; Hnf-3g; HNF3G; Tcf-3g; TCF3G

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UniProt ID: (Human) P55318, (Mouse) P35584

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Entrez Gene ID: (Human) 3171, (Mouse) 15377

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RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding

cellular glucose homeostasis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development spermatogenesis negative regulation of cell proliferation cellular response to starvation anatomical structure morphogenesis chromatin modification cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of hepatocyte differentiation covalent chromatin modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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