Invitrogen

FOXC1 Polyclonal Antibody

FOXC1 Antibody in Immunocytochemistry (ICC/IF)

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FOXC1 Antibody (PA1-807) in ICC/IF

Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody (Product # PA1-807) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a... View More

FOXC1 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

FOXC1 Antibody — Antibody specificity was demonstrated through detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using anti-FOXC1 rabbit polyclonal antibody (catalog # PA1-807), with relevant positive (FGF) and negative target genes/ binding sites(SAT2) {RE}

Product Details

PA1-807

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

ChIP assay (ChIP)

1-3 µg x 10^6 cells

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2247078

Product Specific Information

PA1-807 detects FOXC1 from human and mouse samples.

PA1-807 has been successfully used in Western blot procedures. By Western blot, PA1-807 detects a ~50 kDa band representing FOXC1 from HepG2 cells and mouse kidney cells. This antibody also detects a nonspecific band at ~45 kDa from HepG2 cells, and at ~60 kDa from mouse kidney samples.

The PA1-807 immunogen is a synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.

PA1-807 can be used with blocking peptide PEP-298.

Target Information

FOXC1 is a protein belonging to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this protein is not known, however, it plays a role in the regulation of FGF19-FGFR4-MAPK pathway to promote both the development and maintenance of anterior segment structures within the eye. Mutations in this gene cause various glaucoma, iridogoniodysgenesis anomaly, Peters anomaly that includes central corneal leukoma, absence of the posterior corneal stroma and Descemetmembrane and Axenfeld-Rieger anomaly characterized by posterior corneal embryotoxon, iris adhesion to the Schwalbe line, hypertelorism, hypoplasia of the malar bones, congenital absence of some teeth and mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.

Cancer medicine

Shen Y,Ni S,Li S,Lv B

PA1-807 was used in Western Blotting to analyze stemness-related prognostic genes of CRC based on single-cell RNA-sequencing (scRNA-seq) data.

Mon May 01 00:00:00 UTC 2023

Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.

Cancer medicine

Shen Y,Ni S,Li S,Lv B

PA1-807 was used in Western Blotting to analyze stemness-related prognostic genes of CRC based on single-cell RNA-sequencing (scRNA-seq) data.

Mon May 01 00:00:00 UTC 2023

Single-cell sequencing reveals activation of core transcription factors in PRC2-deficient malignant peripheral nerve sheath tumor.

Cell reports

Zhang X,Lou HE,Gopalan V,Liu Z,Jafarah HM,Lei H,Jones P,Sayers CM,Yohe ME,Chittiboina P,Widemann BC,Thiele CJ,Kelly MC,Hannenhalli S,Shern JF

PA1-807 was used in ChIP assay to define the oncogenic mechanisms underlying PRC2 loss, and discover a two-pronged oncogenic process.

Tue Sep 20 00:00:00 UTC 2022

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

PA1-807 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 UTC 2021

System analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.

Oncotarget

Shang YK,Li C,Liu ZK,Kong LM,Wei D,Xu J,Wang ZL,Bian H,Chen ZN

Published figure using FOXC1 polyclonal antibody (Product # PA1-807) in ChIP assay

Tue Feb 27 00:00:00 UTC 2018

System analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.

Oncotarget

Shang YK,Li C,Liu ZK,Kong LM,Wei D,Xu J,Wang ZL,Bian H,Chen ZN

Published figure using FOXC1 polyclonal antibody (Product # PA1-807) in ChIP assay

Tue Feb 27 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: congenital hydrocephalus; forkhead box C1 protein; Forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7; FREAC-3; HGNC:3800; Mesoderm/mesenchyme forkhead 1; MF-1; myeloid factor-delta; Transcription factor FKH-1

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Gene Aliases: ARA; ch; fkh-1; Fkh1; FKHL7; FOXC1; FREAC-3; FREAC3; frkhda; IGDA; IHG1; IRID1; Mf1; Mf4; RIEG3

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UniProt ID: (Human) Q12948, (Mouse) Q61572

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Entrez Gene ID: (Human) 2296, (Mouse) 17300

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding DNA binding, bending sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

skeletal system development ossification ovarian follicle development eye development ureteric bud development in utero embryonic development somitogenesis lymph vessel development blood vessel remodeling regulation of transcription, DNA-templated transcription from RNA polymerase II promoter Notch signaling pathway brain development heart development cell proliferation germ cell migration mesenchymal cell development neural crest cell development cell migration negative regulation of angiogenesis collagen fibril organization glycosaminoglycan metabolic process lacrimal gland development embryonic heart tube development maintenance of lens transparency vascular endothelial growth factor signaling pathway odontogenesis of dentin-containing tooth camera-type eye development positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter regulation of organ growth vascular endothelial growth factor receptor signaling pathway paraxial mesoderm formation artery morphogenesis regulation of blood vessel size ventricular cardiac muscle tissue morphogenesis cardiac muscle cell proliferation negative regulation of lymphangiogenesis positive regulation of hematopoietic progenitor cell differentiation positive regulation of hematopoietic stem cell differentiation negative regulation of apoptotic process involved in outflow tract morphogenesis blood vessel development kidney development heart morphogenesis transcription, DNA-templated positive regulation of gene expression mesenchymal cell differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXC1 Polyclonal Antibody

FOXC1 Antibody (PA1-807) in ICC/IF

Product Details

PA1-807

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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