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          • Primary Antibodies ›
          • FOXJ1 Antibodies

          Invitrogen

          FOXJ1 Monoclonal Antibody (2A5), eBioscience™

          Advanced Verification
          74 Published Figures
          119 References
          View all (15) FOXJ1 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite FOXJ1 Monoclonal Antibody (2A5), eBioscience™

          • Antibody Testing Data (2)
          • Published Figures (74)
          • Advanced Verification (1)
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 77

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          FOXJ1 Antibody (14-9965-82) in IHC (P)

          Immunohistochemistry of paraffin-embedded mouse lung tissue with Anti-Human/Mouse FOXJ1 Purified followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Avidin HRP (Product # 18-4100-51) (right).Data provided courtesy of Steven L.Brody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry (ICC/IF)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXJ1 Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC, IHC (PFA))
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXJ1 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (IHC)
          FOXJ1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot, ChIP Assay (WB, ChIP)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody in Western Blot (WB)
          FOXJ1 Antibody

          Product Details

          14-9965-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-10 µg/mL
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 62 publications 62 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1-10 µg/mL
          View 6 publications 6 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 3 publications 3 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          Assay-Dependent
          View 33 publications 33 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          ChIP assay (ChIP)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Fruit fly, Human, Mouse, Pig, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2A5

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1548835

          Product Specific Information

          Description: This 2A5 monoclonal antibody reacts with Foxj1, a 50-kDa member of the Forkhead-box (FOX) family of winged-helix transcription factors. Expression of Foxj1 is high in ciliated epithelial cells and low in naive T and B cells, where cellular activation results in downregulation of the factor. Foxj1 modulates germinal center formation and Th1 activation by repressing NFkappaB through IkappaBbeta induction. As such, this transcription factor has been suggested to antagonize autoimmune reactions such as systemic lupus erythematosus and rheumatoid arthritis. In addition, Foxj1 has been linked to the formation of motile cilia in epithelial cells.

          Applications Reported: This 2A5 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

          Applications Tested: This 2A5 antibody has been tested by western blot of mouse tracheal epithelial cells and testes, as well as by immunohistochemistry of mouse lung tissue. This antibody can be used between 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity, through the repression of NF B activity. FOXJ1 also inhibits the humoral immune response in B cells. FOXJ1 deficiency in B cells results in spontaneous and accentuated germinal center formation and is implicated in the development of pathogenic autoantibodies and accentuated responses to immunizations. Abnormal expression of FOXJ1 may be associated with autoimmune diseases and/or other inflammatory diseases. FOXJ1 is also required for cilia formation and left-right axis determination because it increases calpastatin expression, a protein necessary for the ability of basal bodies to anchor to the apical cytoskeleton. FOXJ1 expression may function as an early marker of epithelial cell differentiation, recovery, and function.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: fork head homologue 4; Forkhead box protein J1; forkhead transcription factor HFH-4; forkhead-like 13; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HNF-3/forkhead homolog 4; MGC35202

          View more View less

          Gene Aliases: FKHL-13; FKHL13; FOXJ1; HFH-4; HFH4

          View more View less

          UniProt ID: (Human) Q92949, (Mouse) Q61660

          View more View less

          Entrez Gene ID: (Human) 2302, (Mouse) 15223

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter central tolerance induction negative regulation of germinal center formation positive regulation of central B cell tolerance induction negative regulation of humoral immune response mediated by circulating immunoglobulin transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter humoral immune response cell projection organization actin cytoskeleton organization cell differentiation regulation of epithelial cell differentiation negative regulation of NF-kappaB transcription factor activity negative regulation of T cell differentiation in thymus establishment of apical/basal cell polarity negative regulation of T cell proliferation cilium assembly negative regulation of interleukin-6 biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of B cell activation leukocyte migration cilium morphogenesis left/right pattern formation activation of GTPase activity positive regulation of lung ciliated cell differentiation spermatogenesis determination of left/right symmetry pattern specification process brain development heart development metanephric part of ureteric bud development lung epithelium development epithelium development glomerular parietal epithelial cell development
          It has to be done as per old AB suggested Products section.
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