Invitrogen

FOXL2 Polyclonal Antibody

FOXL2 Antibody in Immunocytochemistry (ICC/IF)

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FOXL2 Antibody (PA1-802) in ICC/IF

Immunofluorescence analysis of FOXL2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXL2 Rabbit Polyclonal Antibody (Product # PA1-802) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at... View More

FOXL2 Antibody in Immunohistochemistry (IHC)

FOXL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FOXL2 Antibody — Antibody specificity was demonstrated through detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using anti-FOXL2 rabbit polyclonal antibody (catalog # PA1-802), with relevant positive (NFATC2) and negative target genes/ binding sites(SAT2) {RE}

Product Details

PA1-802

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

0.2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

ChIP assay (ChIP)

1-3 µg x 10^6 cells

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2278574

Product Specific Information

PA1-802 detects FoxL2 protein in mouse and hamster samples.

PA1-802 has successfully been used in Western blot , immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~39 kDa protein representing FozL2 in CHO whole cell lysate.

The PA1-802 immunogen is a synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2. This sequence is completely conserved in mouse and rat and 92% conserved in human. This peptide (Cat. # PEP-238) is available for use in neutralization and control experiments.

Target Information

FOXL2 is a winged helix/forkhead transcription factor that is highly conserved in human, goat, mouse, and certain aquatic species. Given its cross-species conservation, it appears to play a critical role evolutionarily, likely in ovarian differentiation in mammals and in ovarian somatic cell differentiation and in further follicle development and/or maintenance. Immunohistochemical data shows that FOXL2 is a nuclear protein specifically expressed in eyelids and in fetal and adult ovarian follicular cells. It does not undergo any major posttranslational modifications. FOXL2 is also found to be associated with blepharo-phimosis/ptosis/epicanthus inverses syndrome (BPES). There are two forms of BPES. Type I BPES shows eyelid abnormal-ities that are associated with ovarian failure. In type II BPES, only eyelid defects are found. Studies have shown that FOXL2 is expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Etiology of craniofacial malformations in mouse models of blepharophimosis, ptosis and epicanthus inversus syndrome.

Human molecular genetics

Heude É,Bellessort B,Fontaine A,Hamazaki M,Treier AC,Treier M,Levi G,Narboux-Nême N

PA1-802 was used in immunohistochemistry to elucidate the developmental processes leading to the craniofacial malformations associated with Foxl2 mutations.

Sun Mar 15 00:00:00 UTC 2015

Molecular identification and expression of the Foxl2 gene during gonadal sex differentiation in northern snakehead Channa argus.

Fish physiology and biochemistry

Wang DD,Zhang GR,Wei KJ,Ji W,Gardner JP,Yang RB,Chen KC

PA1-802 was used in immunohistochemistry - paraffin section to investigate sex-specific difference in the growth of Channa argus

Tue Dec 01 00:00:00 UTC 2015

Molecular cloning and analysis of gonadal expression of Foxl2 in the rice-field eel Monopterus albus.

Scientific reports

Hu Q,Guo W,Gao Y,Tang R,Li D

PA1-802 was used in immunohistochemistry - paraffin section to study Foxl2 expression in the Monopterus albus

Mon Nov 03 00:00:00 UTC 2014

Foxl2, a forkhead transcription factor, modulates nonclassical activity of the estrogen receptor-alpha.

Endocrinology

Kim SY,Weiss J,Tong M,Laronda MM,Lee EJ,Jameson JL

PA1-802 was used in western blot to study the role of a forkhead transcription factor Foxl2 in the regulation of estrogen receptor alpha activity.

Sun Nov 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Blepharophimosis; epicanthus inversus; Forkhead box protein L2; forkhead transcription factor FOXL2; LUSTR2; MGC14393; P-Frk; Pituitary forkhead factor; putative transcription factor foxl2

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Gene Aliases: AU045128; BPES; BPES1; FOXL2; PFRK; PINTO; POF3

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UniProt ID: (Human) P58012, (Mouse) O88470

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Entrez Gene ID: (Human) 668, (Mouse) 26927

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding estrogen receptor binding ubiquitin conjugating enzyme binding cysteine-type endopeptidase regulator activity involved in apoptotic process sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter ovarian follicle development extraocular skeletal muscle development apoptotic DNA fragmentation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter female somatic sex determination cell differentiation menstruation positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic eye morphogenesis granulosa cell differentiation oocyte growth single fertilization positive regulation of luteinizing hormone secretion positive regulation of transcription, DNA-templated positive regulation of follicle-stimulating hormone secretion uterus development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXL2 Polyclonal Antibody

FOXL2 Antibody (PA1-802) in ICC/IF

Product Details

PA1-802

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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