OriGene

FOXO1 Monoclonal Antibody (OTI8F12), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

TA810708

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI8F12

Immunogen

Human recombinant protein fragment corresponding to amino acids 356-655 of human FOXO1 produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A

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Gene Aliases: FKH1; FKHR; FOXO1; FOXO1A

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UniProt ID: (Human) Q12778

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Entrez Gene ID: (Human) 2308

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding protein binding beta-catenin binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter blood vessel development temperature homeostasis cellular glucose homeostasis transcription, DNA-templated protein acetylation autophagy apoptotic process cellular response to DNA damage stimulus insulin receptor signaling pathway cellular response to starvation positive regulation of autophagy endocrine pancreas development cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade cellular response to oxidative stress regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter positive regulation of apoptotic process negative regulation of apoptotic process fat cell differentiation negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter enamel mineralization cellular response to hydrogen peroxide cellular response to cold cellular response to hyperoxia cellular response to dexamethasone stimulus cellular response to nitric oxide negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance response to fluoride regulation of neural precursor cell proliferation regulation of reactive oxygen species metabolic process regulation of energy homeostasis
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