Proteintech

FOXO3A Monoclonal Antibody (1F12D11)

FOXO3A Antibody in Immunocytochemistry (ICC/IF)

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FOXO3A Antibody (66428-1-IG) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 66428-1-IG (FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IGG (H+L).

FOXO3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FOXO3A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66428-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:500

View 9 publications 9 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 6 publications 6 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1F12D11

Immunogen

FOXO3A Fusion Protein Ag1289 (325-673 aa encoded by BC021224)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The calculated molecular weight of FOXO3 is 71 kDa, but some experiments using a FOXO3a specific antibody showed the expression of a 90 kDa full-length FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short).

Immunogen sequence: SPIMASTEL DEVQDDDAPL SPMLYSSSAS LSPSVSKPCT VELPRLTDMA GTMNLNDGLT ENLMDDLLDN ITLPPSQPSP TGGLMQRSSS FPYTTKGSGL GSPTSSFNST VFGPSSLNSL RQSPMQTIQE NKPATFSSMS HYGNQTLQDL LTSDSLSHSD VMMTQSDPLM SQASTAVSAQ NSRRNVMLRN DPMMSFAAQP NQGSLVNQNL LHHQHQTQGA LGGSRALSNS VSNMGLSESS SLGSAKHQQQ SPVSQSMQTL SDSLSGSSLY STSANLPVMG HEKFPSDLDL DMFNGSLECD MESIIRSELM DADGLDFNFD SLISTQNVVG LNVGNFTGAK QASSQSWVPG (325-673 aa encoded by BC021224)

Target Information

USP48 is a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of omentin-1 in susceptibility to anxiety and depression like behaviors.

Molecular and cellular endocrinology

Ji L,Zhang L,Liang Z,Zhong S,Liu X,Liu Z,Poon WS,Song Y,Chen B,Wang R

Sun Aug 20 00:00:00 UTC 2023

Ginsenoside Rh2 inhibits CBP/p300-mediated FOXO3a acetylation and epilepsy-induced oxidative damage via the FOXO3a-KEAP1-NRF2 pathway.

European journal of pharmacology

Wu J,Wang S,Zhao W,Li M,Li S

Sun Feb 05 00:00:00 UTC 2023

Ginsenoside Rh2 inhibits CBP/p300-mediated FOXO3a acetylation and epilepsy-induced oxidative damage via the FOXO3a-KEAP1-NRF2 pathway.

European journal of pharmacology

Wu J,Wang S,Zhao W,Li M,Li S

Sun Feb 05 00:00:00 UTC 2023

Ginsenoside Rh2 inhibits CBP/p300-mediated FOXO3a acetylation and epilepsy-induced oxidative damage via the FOXO3a-KEAP1-NRF2 pathway.

European journal of pharmacology

Wu J,Wang S,Zhao W,Li M,Li S

Sun Feb 05 00:00:00 UTC 2023

Ginsenoside Rh2 inhibits CBP/p300-mediated FOXO3a acetylation and epilepsy-induced oxidative damage via the FOXO3a-KEAP1-NRF2 pathway.

European journal of pharmacology

Wu J,Wang S,Zhao W,Li M,Li S

Sun Feb 05 00:00:00 UTC 2023

PARM1 Drives Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension via AKT/FOXO3A Axis.

International journal of molecular sciences

He Z,Chang T,Chen Y,Wang H,Dai L,Zeng H

Tue Mar 28 00:00:00 UTC 2023

The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma.

Autophagy

Niu J,Yan T,Guo W,Wang W,Ren T,Huang Y,Zhao Z,Yu Y,Chen C,Huang Q,Lou J,Guo L

Thu Jun 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

Zhong S,Chen W,Wang B,Gao C,Liu X,Song Y,Qi H,Liu H,Wu T,Wang R,Chen B

Sat Jul 01 00:00:00 UTC 2023

Tumor-Derived Oxidative Stress Triggers Ovarian Follicle Loss in Breast Cancer.

The American journal of pathology

Wu Y,Huang J,Chen H,Tao H,He Y,Yang G,Zha Q,Lash GE,Li P

Mon May 01 00:00:00 UTC 2023

Tumor-Derived Oxidative Stress Triggers Ovarian Follicle Loss in Breast Cancer.

The American journal of pathology

Wu Y,Huang J,Chen H,Tao H,He Y,Yang G,Zha Q,Lash GE,Li P

Mon May 01 00:00:00 UTC 2023

Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis.

Scientific reports

Zhao CQ,Wang C,Liu MM,Cao M,Peng J,Kong DQ,Ren XT,Liu R,Hai CX,Zhang XD

Wed Apr 26 00:00:00 UTC 2023

Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC.

Journal of translational medicine

Du X,Zhang X,Dong J,Zou N,Guo D,Yao W,Wang X,Li S,Song C,Yan K,Shen W,Zhu S

Sat Nov 05 00:00:00 UTC 2022

Inhibition of the cardiac fibroblast-enriched histone methyltransferase Dot1L prevents cardiac fibrosis and cardiac dysfunction.

Cell & bioscience

Xu J,Wang J,Long F,Zhong W,Su H,Su Z,Liu X

Fri Aug 19 00:00:00 UTC 2022

Inhibition of the cardiac fibroblast-enriched histone methyltransferase Dot1L prevents cardiac fibrosis and cardiac dysfunction.

Cell & bioscience

Xu J,Wang J,Long F,Zhong W,Su H,Su Z,Liu X

Fri Aug 19 00:00:00 UTC 2022

FOXO3 Mediates Tooth Movement by Regulating Force-Induced Osteogenesis.

Journal of dental research

Jin A,Hong Y,Yang Y,Xu H,Huang X,Gao X,Gong X,Dai Q,Jiang L

Tue Feb 01 00:00:00 UTC 2022

Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway.

Investigative ophthalmology & visual science

Zhang P,Tian L,Bao J,Li S,Li A,Wen Y,Wang J,Jie Y

Wed Mar 02 00:00:00 UTC 2022

Honokiol improves cognitive impairment in APP/PS1 mice through activating mitophagy and mitochondrial unfolded protein response.

Chemico-biological interactions

Hou M,Bao W,Gao Y,Chen J,Song G

Wed Jan 05 00:00:00 UTC 2022

Estradiol initiates the immune escape of non-small cell lung cancer cells via ERβ/SIRT1/FOXO3a/PD-L1 axis.

International immunopharmacology

Song S,Tang H,Quan W,Shang A,Ling C

Wed Jun 01 00:00:00 UTC 2022

Estradiol initiates the immune escape of non-small cell lung cancer cells via ERβ/SIRT1/FOXO3a/PD-L1 axis.

International immunopharmacology

Song S,Tang H,Quan W,Shang A,Ling C

Wed Jun 01 00:00:00 UTC 2022

Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.

Toxicology and applied pharmacology