Proteintech

FOXP3 Polyclonal Antibody

FIGURE: 1 / 7

FOXP3 Antibody (22228-1-AP) in WB

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

FOXP3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22228-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 17 publications 17 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FOXP3 Fusion Protein Ag17571 (1-330 aa encoded by BC113401)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Immunogen sequence: MPNPRPGKP SAPSLALGPS PGASPSWRAA PKASDLLGAR GPGGTFQGRD LRGGAHASSS SLNPMPPSQL QLPTLPLVMV APSGARLGPL PHLQALLQDR PHFMHQLSTV DAHARTPVLQ VHPLESPAMI SLTPPTTATG VFSLKARPGL PPGINVASLE WVSREPALLC TFPNPSAPRK DSTLSAVPQS SYPLLANGVC KWPGCEKVFE EPEDFLKHCQ ADHLLDEKGR AQCLLQREMV QSLEQQLVLE KEKLSAMQAH LAGKMALTKA SSVASSDKGS CCIVAAGSQG PVVPAWSGPR EAPDSLFAVR RHLWGSHGNS TFPEFLHNMD Y (1-330 aa encoded by BC113401)

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; RGD1562112; scurfin; sf; XPID

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UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371, (Rat) 317382

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding nucleic acid binding DNA binding molecular_function

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Polyclonal Antibody

FOXP3 Antibody (22228-1-AP) in WB

Product Details

22228-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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