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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FOXP3 Antibodies

          Invitrogen

          FOXP3 Monoclonal Antibody (FJK-16s), PE-Cyanine5, eBioscience™

          Advanced Verification
          93 Published Figures
          210 References
          View all (161) FOXP3 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite FOXP3 Monoclonal Antibody (FJK-16s), PE-Cyanine5, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (93)
          • Advanced Verification (1)
          FOXP3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          FOXP3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 95

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          FOXP3 Antibody (15-5773-82) in Flow

          Mouse (BALB/c) splenocytes were surface-stained with Anti-Mouse CD4 FITC (Product # 11-0041-82) and Anti-Mouse CD25 PE (Product # 12-0251-82), and subsequently with Anti-Mouse/Rat Foxp3 PE-Cyanine5 or Rat IgG2a Isotype Control PE-Cyanine5 (Product # 15-4321-80) using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00).The dot plot on the left demonstrates co-staining of CD4 and FJK-16s, while the right plot demonstrates co-staining of CD25 and FJK-16s.Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody

          Product Details

          15-5773-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          1 µg/test
          View 210 publications 210 publications
          Product Specifications

          Species Reactivity

          Bovine, Dog, Cat, Mouse, Pig, Rat

          Published species

          Bovine, Hamster, Mouse, Rat

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          FJK-16s

          Conjugate

          PE-Cyanine5 PE-Cyanine5 PE-Cyanine5
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • Brilliant Violet 421
          • eFluor 450
          • eFluor 506
          • eFluor 570
          • eFluor 615
          • eFluor 660
          • FITC
          • PE 
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          568/666 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_468806

          Product Specific Information

          Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

          Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

          Applications Reported: This FJK-16s antibody has been reported for use in intracellular flow cytometric analysis.

          Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Forkhead box protein P3; forkhead/winged helix transcription factor 3; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

          View more View less

          Gene Aliases: BOS_25920; Foxp3; JM2; RGD1562112; scurfin; sf

          View more View less

          UniProt ID: (Mouse) Q99JB6

          View more View less

          Entrez Gene ID: (Dog) 491876, (Pig) 444998, (Bovine) 506053, (Cat) 100037405, (Mouse) 20371, (Rat) 317382

          View more View less

          Function(s)
          core promoter binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NFAT protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter B cell homeostasis cytokine production myeloid cell homeostasis CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment T cell mediated immunity tolerance induction tolerance induction to self antigen regulation of immunoglobulin production positive regulation of T cell tolerance induction regulation of T cell anergy positive regulation of T cell anergy negative regulation of chronic inflammatory response negative regulation of T cell cytokine production positive regulation of peripheral T cell tolerance induction transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of cell proliferation anatomical structure morphogenesis positive regulation of gene expression negative regulation of histone deacetylation negative regulation of NF-kappaB transcription factor activity negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production negative regulation of CREB transcription factor activity positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation positive regulation of transforming growth factor beta1 production positive regulation of immature T cell proliferation in thymus positive regulation of histone acetylation negative regulation of histone acetylation negative regulation of cytokine biosynthetic process T cell activation negative regulation of T cell proliferation negative regulation of sequence-specific DNA binding transcription factor activity regulatory T cell differentiation negative regulation of interferon-gamma biosynthetic process negative regulation of interleukin-2 biosynthetic process positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of cytokine secretion negative regulation of inflammatory response negative regulation of immune response T cell receptor signaling pathway negative regulation of T-helper 17 cell differentiation biological_process response to virus
          It has to be done as per old AB suggested Products section.
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