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          • Primary Antibodies ›
          • FOXP3 Antibodies
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          Invitrogen

          FOXP3 Monoclonal Antibody (FJK-16s), eFluor™ 506, eBioscience™

          Advanced Verification
          88 Published Figures
          264 References
          View all (171) FOXP3 antibodies

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          Datasheet
          Protocols
          Datasheet
          Protocols

          Cite FOXP3 Monoclonal Antibody (FJK-16s), eFluor™ 506, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (88)
          • Advanced Verification (1)
          FOXP3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          FOXP3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 90

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          FOXP3 Antibody (69-5773-80) in Flow

          Staining of BALB/c splenocytes with Anti-Mouse CD4 APC (Product # 17-0042-82) (left), Anti-Mouse CD25 PE (Product # 12-0251-82) (right), followed by intracellular staining with 0.25 µg of Anti-Mouse/Rat Foxp3 eFluor® 506, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          FOXP3 Antibody in Flow Cytometry (Flow)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry (ICC/IF)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
          FOXP3 Antibody in Immunocytochemistry (ICC/IF)
          FOXP3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          FOXP3 Antibody in Immunocytochemistry (ICC/IF)
          FOXP3 Antibody in Immunocytochemistry (ICC/IF)
          FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          FOXP3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody in Immunohistochemistry (IHC)
          FOXP3 Antibody

          Product Details

          69-5773-80

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 70 publications 70 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 7 publications 7 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 180 publications 180 publications

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          ChIP assay (ChIP)

          -
          View 2 publications 2 publications

          In Situ Hybridization (ISH) (ISH)

          -
          View 1 publication 1 publication
          Product Specifications

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 506, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          FJK-16s

          Conjugate

          eFluor™ 506 eFluor™ 506 eFluor™ 506
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • Brilliant Violet 421
          • eFluor 450
          • eFluor 570
          • eFluor 615
          • eFluor 660
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          419/508 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Wet ice

          RRID

          AB_2637366

          Product Specific Information

          Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

          Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

          Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to BestProtocols® for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

          Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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