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Invitrogen
FPGS Recombinant Rabbit Monoclonal Antibody (7E2H1)
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FIGURE: 1 / 1
FPGS Antibody (MA5-55448) in WB
Western Blot analysis of various lysates using FPGS Recombinant Monoclonal Antibody (Product # MA5-55448) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-55448
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7E2H1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 450-587 of human FPGS.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667689
Product Specific Information
Immunogen Sequence: NADQQNFTVT LDQVLLRCLE HQQHWNHLDE EQASPDLWSA PSPEPGGSAS LLLAPHPPHT CSASSLVFSC ISHALQWISQ GRDPIFQPPS PPKGLLTHPV AHSGASILRE AAAIHVLVTG SLHLVGGVLK LLEPALSQ
Target Information
Folylpoly-gamma-glutamate synthetase (FPGS) catalyzes the addition of several equivalents of glutamic acid to the gamma-carboxyl group in the side chain of folate cofactors and analogs. Folylpoly-gamma-glutamate synthetase has three functions in folate homeostasis in mammals: polyglutamation prevents efflux of folate cofactors from the cell, it increases the binding of folate cofactors to some of the enzymes of folate interconversion and biosynthesis, and it appears to allow the accumulation of folates in the mitochondria that are required for glycine synthesis. Folylpoly-gamma-glutamate synthetase is widely expressed in human tumors and is tightly linked either to proliferation or to a lack of differentiation. The cytotoxicity of both thymidylate synthase and purine inhibitors requires continued inhibition of target for greater than one generation time, so that the integrative function of FPGS adds considerably to the efficiency of folate antimetabolites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Folylpoly-gamma-glutamate synthetase; Folylpolyglutamate synthase, mitochondrial; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase
Gene Aliases: FPGS
UniProt ID: (Human) Q05932
Entrez Gene ID: (Human) 2356, (Rat) 687266
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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