Proteintech

FSTL1 Polyclonal Antibody

FSTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FSTL1 Antibody (20182-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 20182-1-AP ( FSTL1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FSTL1 Antibody in Immunoprecipitation (IP)

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Product Details

20182-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FSTL1 Fusion Protein Ag14057 (1-308 aa encoded by BC000055)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MWKRWLALA LALVAVAWVR AEEELRSKSK ICANVFCGAG RECAVTEKGE PTCLCIEQCK PHKRPVCGSN GKTYLNHCEL HRDACLTGSK IQVDYDGHCK EKKSVSPSAS PVVCYQSNRD ELRRRIIQWL EAEIIPDGWF SKGSNYSEIL DKYFKNFDNG DSRLDSSEFL KFVEQNETAI NITTYPDQEN NKLLRGLCVD ALIELSDENA DWKLSFQEFL KCLNPSFNPP EKKCALEDET YADGAETEVD CNRCVCACGN WVCTAMTCDG KNQKGAQTQT EEEMTRYVQE LQKHQETAEK TKRVSTKEI (1-308 aa encoded by BC000055 )

Target Information

Follistatin-like protein 1 (FSTL1) is a widely-expressed, extracellular glycoprotein that is homologously grouped into the osteonectin (BM-40/SPARC) family of secreted proteins based on its possession of both a follistatin-like and extracellular calcium-binding domain. Initially identified as a TGF-beta-inducible protein in a cloned mouse osteoblast cell line, FSTL1 has since been implicated in an array of cell-type-specific functions, such as the regulation of proliferation, differentiation, apoptosis and migration, as well as a number of biological processes, including embryonic development, inflammatory response, angiogenesis, tumorigenesis, and immune disease pathogenesis. Highly conserved across mammalian species and widely expressed in human tissues, FSTL1 can be upregulated through signaling mediators of the innate immune system,such as TLR4 agonists and the arthritogenic cytokine IL-1beta via NFkappaB pathways, to stimulate the expression and secretion of pro-inflammatory cytokines, including TNF-alpha, IL-1beta, IL-6 and IL-8. While cells of mesenchymal lineage are capable of FSTL1 production, FSTL1 expression is notably absent from cells of hematopoietic lineage under normal physiological conditions. Macrophages and monocytes are, however, capable of taking up FSTL1 at sites of inflammation where FSTL1 stimulation can cause the expression of caspase-1 and its resultant enzymatic cleavage of active IL-1beta from pro-IL-1beta. Whereas the overexpression of FSTL1 has been noted as a substantial contributor to the progression of immune diseases like rheumatoid arthritis (RA) and osteoarthritis (OA), diminished FSTL1 serum levels have been identified as playing a significant part in both ovarian and endometrial carcinogenesis, where it directly affects cell proliferation, migration and invasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx^-/- mice.

Bone research

Lin J,Yang Y,Zhou W,Dai C,Chen X,Xie Y,Han S,Liu H,Hu Y,Tang C,Bunpetch V,Zhang D,Chen Y,Zou X,Chen D,Liu W,Ouyang H

Fri Jan 07 00:00:00 UTC 2022

International immunopharmacology

Liu F,Zhang J,Zhang D,Qi Q,Cui W,Pan Y,Liu X,Xu J,Qiao X,Wang Z,Dong L

Mon Aug 01 00:00:00 UTC 2022

Prediction of Prognosis and Recurrence of Bladder Cancer by ECM-Related Genes.

Journal of immunology research

Zhao H,Chen Z,Fang Y,Su M,Xu Y,Wang Z,Gyamfi MA,Zhao J

Mon Apr 25 00:00:00 UTC 2022

Follistatin-like 1 deficiency impairs T cell development to promote lung metastasis of triple negative breast cancer.

Aging

Ma J,Yang Y,Wang L,Jia X,Lu T,Zeng Y,Liu L,Gao Y

Fri Feb 26 00:00:00 UTC 2021

FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival.

American journal of cancer research

Wu M,Ding Y,Wu N,Jiang J,Huang Y,Zhang F,Wang H,Zhou Q,Yang Y,Zhuo W,Teng L

Fri Apr 02 00:00:00 UTC 2021

FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival.

American journal of cancer research

Wu M,Ding Y,Wu N,Jiang J,Huang Y,Zhang F,Wang H,Zhou Q,Yang Y,Zhuo W,Teng L

Fri Apr 02 00:00:00 UTC 2021

Mesenchymal stem cells induce tumor stroma formation and epithelial‑mesenchymal transition through SPARC expression in colorectal cancer.

Oncology reports

Naito T,Yuge R,Kitadai Y,Takigawa H,Higashi Y,Kuwai T,Kuraoka K,Tanaka S,Chayama K

Tue Jun 01 00:00:00 UTC 2021

Follistatin-like Protein 1 Inhibits Lung Cancer Metastasis by Preventing Proteolytic Activation of Osteopontin.

Cancer research

Chiou J,Chang YC,Tsai HF,Lin YF,Huang MS,Yang CJ,Hsiao M

Sun Dec 15 00:00:00 UTC 2019

FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway.

Cell death & disease