Invitrogen

FXR Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-16822

Applications
Tested Dilution
Publications

Western Blot (WB)

1-4 µg/mL
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to the C-terminus of human FXR.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2155056

Product Specific Information

Suggested positive control: HeLa nuclear extract.

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

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Gene Aliases: AI957360; BAR; FXR; HRR-1; HRR1; NR1H4; RIP14; Rxrip14

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UniProt ID: (Human) Q96RI1, (Mouse) Q60641

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Entrez Gene ID: (Human) 9971, (Mouse) 20186

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Function(s)
RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding retinoid X receptor binding chenodeoxycholic acid binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding double-stranded DNA binding receptor activity metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter nitrogen catabolite activation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter Notch signaling pathway bile acid metabolic process intracellular receptor signaling pathway regulation of urea metabolic process intracellular bile acid receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter negative regulation of bile acid biosynthetic process cellular response to organonitrogen compound cellular response to bile acid positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle transcription initiation from RNA polymerase II promoter inflammatory response signal transduction response to glucose regulation of low-density lipoprotein particle clearance bile acid and bile salt transport response to lipopolysaccharide toll-like receptor 4 signaling pathway histone H3-R17 methylation cellular triglyceride homeostasis bile acid signaling pathway negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response digestive tract development regulation of insulin secretion involved in cellular response to glucose stimulus regulation of bile acid biosynthetic process cellular response to fatty acid interleukin-17 secretion regulation of cholesterol metabolic process negative regulation of interferon-gamma secretion negative regulation of tumor necrosis factor secretion
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