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FZD8 Antibody (PA5-120635) in WB
Western blot analysis of FZD8 in extracts of various cell lines. Samples were incubated with FZD8 Polyclonal antibody (Product # PA5-120635) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120635
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 130-275 of human FZD8 (NP_114072.1).
Conjugate
Form
Liquid
Concentration
0.96 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914207
Product Specific Information
Positive test controls include: 293T, A-549, HeLa, Mouse liver, Rat liver.
Immunogen sequence: PDRMRCDRLP EQGNPDTLCM DYNRTDLTTA APSPPRRLPP PPPGEQPPSG SGHGRPPGAR PPHRGGGRGG GGGDAAAPPA RGGGGGGKAR PPGGGAAPCE PGCQCRAPMV SVSSERHPLY NRVKTGQIAN CALPCHNPFF SQDERA
Target Information
Members of the Frizzled family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Frizzled 8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. Its expression has been reported in brain, heart, kidney, pancreas, and skeletal muscle. ESTs have been isolated from embryo, eye, head/neck, heart, kidney, liver/spleen, pancreas, prostate, and testis libraries, as well as many tumor libraries. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: frizzled 8, seven transmembrane spanning receptor; frizzled family receptor 8; frizzled homolog 8; Frizzled-8; Fz-8; HGNC:4046
Gene Aliases: FZ-8; Fz8; FZD8; hFZ8
UniProt ID: (Human) Q9H461, (Mouse) Q61091, (Rat) Q498S8
Entrez Gene ID: (Human) 8325, (Mouse) 14370, (Rat) 364754
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
positive regulation of protein phosphorylation
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
multicellular organism development
Wnt signaling pathway
T cell differentiation in thymus
non-canonical Wnt signaling pathway
positive regulation of JUN kinase activity
positive regulation of transcription from RNA polymerase II promoter
canonical Wnt signaling pathway
neuron differentiation
biological_process
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