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Invitrogen
Factor IX Recombinant Rabbit Monoclonal Antibody (034)
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Factor IX Antibody (MA5-29254) in WB
Western Blot using Factor IX Recombinant Rabbit Monoclonal Antibody (34) (Product # MA5-29254) at 1:500 dilution. Lane A: A431 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 55 kDa.
Product Details
MA5-29254
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
034
Immunogen
Recombinant Human Coagulation Factor IX/FIX/F9 protein (Met1-Thr461)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785154
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Coagulation Factor IX/FIX/F9.
Target Information
Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is approximately 3 µg/mL. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Christmas factor; Coagulation factor IX; EC 3.4.21.22; factor 9; factor IX F9; Factor9; FIX F9; plasma thromboplastic component; Plasma thromboplastin component; PTC; RP6-88D7.1; serine protease
Gene Aliases: F9; F9 p22; FIX; HEMB; P19; PTC; THPH8
UniProt ID: (Human) P00740
Entrez Gene ID: (Human) 2158
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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