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Invitrogen
Factor VII Polyclonal Antibody
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FIGURE: 1 / 1
Factor VII Antibody (PA5-47475) in WB
Western blot analysis of Factor VII in mouse placenta tissue. Samples were incubated in Factor VII polyclonal antibody (Product # PA5-47475) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Coagulation Factor VII at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47475
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant mouse Coagulation Factor VII Ala42-Leu446
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608292
Product Specific Information
In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Factor VII, rhFactor X, and rhFactor Xa is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Coagulation factor VII is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in F7 production can cause coagulopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coagulation factor VII; Factor 7; Factor7; Serum prothrombin conversion accelerator
Gene Aliases: AI132620; Cf7; F7; FVII
UniProt ID: (Mouse) P70375
Entrez Gene ID: (Mouse) 14068
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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