Invitrogen

FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™

FceR1 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

FceR1 alpha Antibody (14-5899-80) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (green histogram) or Anti-Human Fc epsilon Receptor I a (FcepsilonR1) Purified (blue histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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Product Details

14-5899-80

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 5 publications 5 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Published species

Human, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

AER-37 (CRA1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_467709

Product Specific Information

Description: The AER-37 monoclonal antibody reacts with the FcεRIalpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIalpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIalpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: The AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. It has been reported to not block IgE binding.

Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera.

Diagnostics (Basel, Switzerland)

Prakash PS,Barwary NJS,Weber MHW,Wan D,Conejeros I,Pereira Moreira B,Alharbi WS,van Hellemond JJ,Akinwale J,Falcone FH

Published figure using FceR1 alpha monoclonal antibody (Product # 14-5899-82) in Flow Cytometry

Thu Aug 25 00:00:00 UTC 2022

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

14-5899-82 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 UTC 2021

AllergoOncology: Generating a canine anticancer IgE against the epidermal growth factor receptor.

The Journal of allergy and clinical immunology

Fazekas-Singer J,Singer J,Ilieva KM,Matz M,Herrmann I,Spillner E,Karagiannis SN,Jensen-Jarolim E

14-5899-82 was used in Flow Cytometry to evaluate the cytotoxic killing potency of can225IgE-l by MPT-1 canine mast cells against A431 and measured specific tumor cell killing.

Sat Sep 01 00:00:00 UTC 2018

Human basophils are a source of - and are differentially activated by - IL-31.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Raap U,Gehring M,Kleiner S,Rüdrich U,Eiz-Vesper B,Haas H,Kapp A,Gibbs BF

14589982 was used in flow cytometry to assess the functional role of IL-31 in basophil biology

Sat Apr 01 00:00:00 UTC 2017

Clinical efficacy of omalizumab in chronic spontaneous urticaria is associated with a reduction of FcεRI-positive cells in the skin.

Theranostics

Metz M,Staubach P,Bauer A,Brehler R,Gericke J,Kangas M,Ashton-Chess J,Jarvis P,Georgiou P,Canvin J,Hillenbrand R,Erpenbeck VJ,Maurer M

14-5899 was used in Immunohistochemistry to discover that treatment with omalizumab was associated with reduction in Fc?RI+and IgE+basophils and intradermal cells.

Fri Jan 05 00:00:00 UTC 2018

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.

The Journal of experimental medicine

Henry EK,Sy CB,Inclan-Rico JM,Espinosa V,Ghanny SS,Dwyer DF,Soteropoulos P,Rivera A,Siracusa MC

14589982 was used in flow cytometry to find a role for Car enzymes in regulating mast cell lineage commitment

Mon Aug 22 00:00:00 UTC 2016

Redefinition of the human mast cell transcriptome by deep-CAGE sequencing.

Blood

Motakis E,Guhl S,Ishizu Y,Itoh M,Kawaji H,de Hoon M,Lassmann T,Carninci P,Hayashizaki Y,Zuberbier T,Forrest AR,Babina M

14-5899-82 was used in Functional assay to characterise in detail the human mast cell transcriptome.

Thu Apr 24 00:00:00 UTC 2014

NADPH oxidase-independent formation of extracellular DNA traps by basophils.

Journal of immunology (Baltimore, Md. : 1950)

Morshed M,Hlushchuk R,Simon D,Walls AF,Obata-Ninomiya K,Karasuyama H,Djonov V,Eggel A,Kaufmann T,Simon HU,Yousefi S

14589982 was used in immunocytochemistry to propose that basophils exert direct innate immune effector functions in the extracellular space

Sun Jun 01 00:00:00 UTC 2014

Interferons modulate Fc epsilon RI-dependent production of autoregulatory IL-10 by circulating human monocytoid dendritic cells.

The Journal of allergy and clinical immunology

Le T,Tversky J,Chichester KL,Bieneman AP,Huang SK,Wood RA,Schroeder JT

14-5899-82 was used in Neutralization experiments to characterise Fc epsilon receptor for IgE-dependent cytokine responses and their regulation in circulating human blood monocytoid dendritic cells.

Thu Jan 01 00:00:00 UTC 2009

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience™

FceR1 alpha Antibody (14-5899-80) in Flow

Product Details

14-5899-80

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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