Invitrogen

FceR1 alpha Monoclonal Antibody (MAR-1), PE-Cyanine7, eBioscience™

13 Published Figures

16 References

View all (78) FceR1 alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (13)

FceR1 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

FceR1 alpha Antibody (25-5898-82) in Flow

Staining of MC/9 cell line (a mouse mast cell line) with 0.03 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888-82) (blue histogram) or 0.03 µg of Anti-Mouse Fc epsilon Receptor I alpha (FceR1) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

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Product Details

25-5898-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 15 publications 15 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAR-1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573493

Product Specific Information

Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Tue Jan 18 00:00:00 UTC 2022

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

The Journal of biological chemistry

Cardenas RA,Gonzalez R,Sanchez E,Ramos MA,Cardenas EI,Rodarte AI,Alcazar-Felix RJ,Isaza A,Burns AR,Heidelberger R,Adachi R

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Mon Aug 02 00:00:00 UTC 2021

Tespa1 plays a role in the modulation of airway hyperreactivity through the IL-4/STAT6 pathway.

Journal of translational medicine

Yang R,Wang G,Li L,He H,Zheng M,Lu L,Wu S

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Mon Nov 23 00:00:00 UTC 2020

Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells.

Journal of neuroinflammation

Pérez-Rodríguez MJ,Ibarra-Sánchez A,Román-Figueroa A,Pérez-Severiano F,González-Espinosa C

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Fri Mar 27 00:00:00 UTC 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

25-5898 was used in flow cytometry to analyse the myeloid populations in various mouse tissues by flow cytometry.

Fri Jun 19 00:00:00 UTC 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

25-5898 was used in flow cytometry to analyse the myeloid populations in various mouse tissues by flow cytometry.

Fri Jun 19 00:00:00 UTC 2020

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

25-5898 was used in Flow cytometry/Cell sorting to identify a discrete ILC2-committed population and delineated transition states between early progenitors and a highly heterogeneous ILC1, ILC3, and NK precursor cell cluster.

Tue Jul 16 00:00:00 UTC 2019

Type I interferon limits mast cell-mediated anaphylaxis by controlling secretory granule homeostasis.

PLoS biology

Kobayashi T,Shimabukuro-Demoto S,Tsutsui H,Toyama-Sorimachi N

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Fri Nov 01 00:00:00 UTC 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 UTC 2019

Early Resistance of Non-virulent Mycobacterial Infection in C57BL/6 Mice Is Associated With Rapid Up-Regulation of Antimicrobial Cathelicidin Camp.

Frontiers in immunology

Adam L,López-González M,Björk A,Pålsson S,Poux C,Wahren-Herlenius M,Fernández C,Spetz AL

25-5898 was used in Flow cytometry/Cell sorting to assess the early innate immune events locally in the lungs to identify factors that may contribute to the control of non-virulent mycobacterial burden.

Wed Sep 18 00:00:00 UTC 2019

Type I IFNs Are Required to Promote Central Nervous System Immune Surveillance through the Recruitment of Inflammatory Monocytes upon Systemic Inflammation.

Frontiers in immunology

Peralta Ramos JM,Bussi C,Gaviglio EA,Arroyo DS,Baez NS,Rodriguez-Galan MC,Iribarren P

Wed Nov 20 00:00:00 UTC 2019

Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life.

Mucosal immunology

Patel KR,Aven L,Shao F,Krishnamoorthy N,Duvall MG,Levy BD,Ai X

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Sun May 01 00:00:00 UTC 2016

Isolation of Mature (Peritoneum-Derived) Mast Cells and Immature (Bone Marrow-Derived) Mast Cell Precursors from Mice.

PloS one

Meurer SK,Neß M,Weiskirchen S,Kim P,Tag CG,Kauffmann M,Huber M,Weiskirchen R

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Mon Jul 24 00:00:00 UTC 2017

Rhinovirus-induced IL-25 in asthma exacerbation drives type 2 immunity and allergic pulmonary inflammation.

Science translational medicine

Beale J,Jayaraman A,Jackson DJ,Macintyre JDR,Edwards MR,Walton RP,Zhu J,Man Ching Y,Shamji B,Edwards M,Westwick J,Cousins DJ,Yi Hwang Y,McKenzie A,Johnston SL,Bartlett NW

25-5898 was used in Flow cytometry/Cell sorting to demonstrate asthmatic epithelial cells have an increased intrinsic capacity for expression of a pro-type 2 cytokine in response to a viral infection.

Wed Oct 01 00:00:00 UTC 2014

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

Wed Jul 01 00:00:00 UTC 2009

IL-21R is essential for epicutaneous sensitization and allergic skin inflammation in humans and mice.

The Journal of clinical investigation

Jin H,Oyoshi MK,Le Y,Bianchi T,Koduru S,Mathias CB,Kumar L,Le Bras S,Young D,Collins M,Grusby MJ,Wenzel J,Bieber T,Boes M,Silberstein LE,Oettgen HC,Geha RS

Published figure using FceR1 alpha monoclonal antibody (Product # 25-5898-82) in Flow Cytometry

Thu Jan 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3

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Gene Aliases: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5

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UniProt ID: (Mouse) P20489

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Entrez Gene ID: (Mouse) 14125

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Function(s)

protein binding IgE receptor activity IgE binding

Process(es)

activation of MAPK activity positive regulation of type I hypersensitivity serotonin secretion signal transduction cell surface receptor signaling pathway activation of JUN kinase activity leukotriene biosynthetic process Fc-epsilon receptor signaling pathway positive regulation of mast cell degranulation positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FceR1 alpha Monoclonal Antibody (MAR-1), PE-Cyanine7, eBioscience™

FceR1 alpha Antibody (25-5898-82) in Flow

Product Details

25-5898-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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