Invitrogen

Fetal Hemoglobin Monoclonal Antibody (HBF-1), FITC

Product Details

MHFH01

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HBF-1

Immunogen

Human Fetal Hemoglobin (HbF)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA, <10% sucrose

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10375007

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

This Fetal Hemoglobin monoclonal antibody specifically recognizes fetal hemoglobin found in fetal erythrocytes.

Target Information

Hemorrhage of cells from an Rh+ fetus into the circulation of an Rh- mother may result in the formation of Rh-reactive antibodies in the mother. Rh hemolytic anemia in a subsequent Rh+ fetus may result from placental transfer of antibodies formed in the mother to the fetus. Although the volume of fetal erythrocytes found in the maternal circulation during pregnancy and immediately post-partum is reported to be small and of uncertain clinical significance in many cases, substantial hemorrhage may result from a number of causes including fetal or maternal trauma and placental defects. Erythrocytes containing fetal hemoglobin may be found in individuals of any age, but with lower amounts of fetal hemoglobin compared to fetal red cells. These cells have been termed F cells. High levels of F cells may also exist in adults with a heterogeneous group of genetic disorders of uncertain etiology, referred to as Hereditary Persistence of Fetal Hemoglobin. Other clinical conditions causing significant levels of anemia may also result in elevated levels of F cells. Several clinical conditions have been described with increased levels of F cells. These conditions include hereditary anemic diseases such as sickle cell anemia and thalassemia major.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The protective effect of the spleen in sickle cell patients. A comparative study between patients with asplenia/hyposplenism and hypersplenism.

Frontiers in physiology

Peretz S,Livshits L,Pretorius E,Makhro A,Bogdanova A,Gassmann M,Koren A,Levin C

MHFH01 was used in Flow cytometry/Cell sorting to evaluate the association between the spleen status with clinical and laboratory parameters in 31 SCD patients: Group a) Patients with asplenia/hyposplenism (N = 22) (including auto-splenectomy and splenectomized patients) vs. Group b) patients with splenomegaly and or hypersplenism (N = 9).

Sat Sep 17 00:00:00 UTC 2022

ZNF410 represses fetal globin by singular control of CHD4.

Nature genetics

Vinjamur DS,Yao Q,Cole MA,McGuckin C,Ren C,Zeng J,Hossain M,Luk K,Wolfe SA,Pinello L,Bauer DE

MHFH01 was used in Flow cytometry/Cell sorting to identify zinc-finger protein (ZNF) 410 as an fetal hemoglobin repressor.

Sat May 01 00:00:00 UTC 2021

BMI1 enables extensive expansion of functional erythroblasts from human peripheral blood mononuclear cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Liu S,Wu M,Lancelot M,Deng J,Gao Y,Roback JD,Chen T,Cheng L

MHFH01 was used in Flow Cytometry to report that ectopic expression of the human BMI1 gene confers extensive expansion of human erythroblasts, which can be derived readily from adult peripheral blood mononuclear cells of either healthy donors or sickle cell patients.

Wed May 05 00:00:00 UTC 2021

Hemoglobin F mitigation of sickle cell complications decreases with aging.

American journal of hematology

Tolu SS,Reyes-Gil M,Ogu UO,Thomas M,Bouhassira EE,Minniti CP

MHFH01 was used in Flow cytometry/Cell sorting to show that hemoglobin F mitigation of sickle cell complications decreases with aging.

Fri May 01 00:00:00 UTC 2020

Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.

JCI insight

Krishnamoorthy S,Pace B,Gupta D,Sturtevant S,Li B,Makala L,Brittain J,Moore N,Vieira BF,Thullen T,Stone I,Li H,Hobbs WE,Light DR

MHFH01 was used in Flow Cytometry to investigate the ability of dimethyl fumarate to activate γ-globin transcription, and enhance foetal haemoglobin.

Thu Oct 19 00:00:00 UTC 2017

Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.

PloS one

Shearstone JR,Golonzhka O,Chonkar A,Tamang D,van Duzer JH,Jones SS,Jarpe MB

MHFH01 was used in flow cytometry to demonstrate that chemical inhibition of HDAC1/2 induces gamma-globin and propose a role for the GATA2 gene in this process

Tue Aug 30 00:00:00 UTC 2016

Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cells.

Journal of biomolecular screening

Li H,Xie W,Gore ER,Montoute MN,Bee WT,Zappacosta F,Zeng X,Wu Z,Kallal L,Ames RS,Pope AJ,Benowitz A,Erickson-Miller CL

MHFH01 was used in flow cytometry to develop a method using primary human bone marrow-derived erythroid progenitor cells to screen for compounds that induce γ-globin production.

Sun Dec 01 00:00:00 UTC 2013

Abstracts of the 16th meeting of the Clinical Cytometry Society. Clinical applications of cytometry. November 11-13, 2001. Orlando, Florida, USA.

Cytometry

No authors listed

MHFH01 was used in Flow cytometry/Cell sorting to compare CD38 (AB/C) values on CD8 T cells when a combined gate (lymphocytes and CD31 T cells) or a single gate (CD31 T cells) were applied.

Mon Oct 15 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: A-gamma globin; alpha-1 globin; gamma A hemoglobin; gamma globin; Gamma-1-globin; Hb F Agamma; hemoglobin alpha 1; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain; Hemoglobin subunit gamma-1; hemoglobin, gamma A; hemoglobin, gamma, regulator of

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Gene Aliases: HBG-T2; HBG1; HBGA; HBGR; HSGGL1; PRO2979

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UniProt ID: (Human) P69891

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Entrez Gene ID: (Human) 3047

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Function(s)

oxygen transporter activity iron ion binding oxygen binding heme binding

Process(es)

blood coagulation oxygen transport

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Fetal Hemoglobin Monoclonal Antibody (HBF-1), FITC

Product Details

MHFH01

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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