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Invitrogen
Fibrinogen gamma Monoclonal Antibody (5H9)
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FIGURE: 1 / 3
Fibrinogen gamma Antibody (MA5-49169) in ICC/IF
Immunocytochemistry analysis of Fibrinogen gamma in HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Fibrinogen gamma Monoclonal antibody (Product # MA5-49169) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilu... View More
Product Details
MA5-49169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
5H9
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FGG (133-163aa IRYLQEIYNSNNQKIVNLKEKVAQLEAQCQE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074827
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence is different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids.
Positive Control - WB: human HepG2 whole cell, human HepG2 whole cell. ICC/IF: HEP3B cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fibg; Fibrinogen gamma chain; fibrinogen, gamma polypeptide; testicular tissue protein Li 70
Gene Aliases: FGG; PRO2061
UniProt ID: (Human) P02679
Entrez Gene ID: (Human) 2266
platelet degranulation
cell-matrix adhesion
signal transduction
blood coagulation
protein secretion
extracellular matrix organization
plasminogen activation
positive regulation of heterotypic cell-cell adhesion
fibrinolysis
cellular protein complex assembly
positive regulation of vasoconstriction
positive regulation of exocytosis
positive regulation of protein secretion
protein polymerization
response to calcium ion
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
blood coagulation, fibrin clot formation
positive regulation of peptide hormone secretion
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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