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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Fibronectin Antibodies

          Proteintech

          Fibronectin Polyclonal Antibody

          578 References
          View all (99) Fibronectin antibodies

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          Datasheet
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          Cite Fibronectin Polyclonal Antibody

          • Antibody Testing Data (11)
          Fibronectin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Fibronectin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          Fibronectin Antibody (15613-1-AP) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (Product # 15613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Fibronectin Antibody in Immunocytochemistry (ICC/IF)
          Fibronectin Antibody in Immunocytochemistry (ICC/IF)
          Fibronectin Antibody in Immunocytochemistry (ICC/IF)
          Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Fibronectin Antibody in Western Blot (WB)
          Fibronectin Antibody in Western Blot (WB)
          Fibronectin Antibody in Western Blot (WB)
          Fibronectin Antibody in Immunoprecipitation (IP)

          Product Details

          15613-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 384 publications 384 publications

          Immunohistochemistry (IHC)

          -
          View 83 publications 83 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 100 publications 100 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications

          Not applicable (N/A)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Fibronectin Fusion Protein Ag8004 (2177-2386 aa encoded by BC005858)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.28 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: DQQRHKVRE EVVTVGNSVN EGLNQPTDDS CFDPYTVSHY AVGDEWERMS ESGFKLLCQC LGFGSGHFRC DSSRWCHDNG VNYKIGEKWD RQGENGQMMS CTCLGNGKGE FKCDPHEATC YDDGKTYHVG EQWQKEYLGA ICSCTCFGGQ RGWRCDNCRR PGGEPSPEGT TGQSYNQYSQ RYHQRTNTNV NCPIECFMPL DVQADREDSR E (2177-2386 aa encoded by BC005858)

          Target Information

          Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor

          View more View less

          Gene Aliases: CIG; E330027I09; ED-B; FIBNEC; FINC; FN; Fn-1; FN1; FNZ; GFND; GFND2; LETS; MSF

          View more View less

          UniProt ID: (Human) Q585T2, (Mouse) P11276

          View more View less

          Entrez Gene ID: (Human) 2335, (Mouse) 14268, (Rat) 25661

          View more View less

          Function(s)
          protease binding integrin binding protein binding collagen binding heparin binding peptidase activator activity identical protein binding mercury ion binding extracellular matrix structural constituent
          Process(es)
          angiogenesis cell activation acute-phase response cell-substrate junction assembly cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion glial cell migration regulation of cell shape positive regulation of peptidase activity peptide cross-linking extracellular matrix organization positive regulation of cell migration integrin activation substrate adhesion-dependent cell spreading endodermal cell differentiation wound healing negative regulation of apoptotic process positive regulation of axon extension positive regulation of chemotaxis cellular response to mercury ion cellular response to interleukin-1 regulation of protein phosphorylation platelet degranulation response to ischemia positive regulation of cell proliferation response to wounding response to ozone positive regulation of gene expression extracellular matrix disassembly cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus positive regulation of fibroblast proliferation leukocyte migration response to glucocorticoid regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to glucose stimulus cellular response to prostaglandin E stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of transforming growth factor-beta secretion ossification
          It has to be done as per old AB suggested Products section.
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