Invitrogen

Fibronectin Polyclonal Antibody

Fibronectin Antibody in Western Blot (WB)

FIGURE: 1 / 23

Fibronectin Antibody (PA5-29578) in WB

Knockout of Fibronectin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR616644_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Fibronectin was performed by loading 30 µg of Hep G2 Wild Type (Lane 1), Hep G2 Wild Type treated with 1X PTI for 4hrs (Lane 2),Hep G2 Cas9 (Lane 3), Hep G2 Cas9 treated with 1X PTI for 4hrs (Lane 4), Hep G2 Fibronectin KO (Lane 5) and Hep G2 Fibronectin KO treated with 1X PTI for 4hrs (Lane 6) whole cell extracts. The s... View More

Fibronectin Antibody in Immunocytochemistry (ICC/IF)

Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibronectin Antibody in Immunoprecipitation (IP)

Fibronectin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-29578

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

1:1000-1:10000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547054

Product Specific Information

Recommended positive controls: HepG2, HepG2(10 mM Nicotinamide and 0.4 mM Trichostatin A treatment for 48 hr), HeLa mock and shFN1, mouse plasma.

Predicted reactivity: Mouse (92%), Rat (92%), Chicken (88%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

PA5-29578 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to find an increased expression of the mesenchymal markers a-SMA and fibronectin in TFCs in the thyroid glands of AITD patients.

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Wed Feb 08 00:00:00 UTC 2023

CHD-associated enhancers shape human cardiomyocyte lineage commitment.

eLife

Armendariz DA,Goetsch SC,Sundarrajan A,Sivakumar S,Wang Y,Xie S,Munshi NV,Hon GC

PA5-29578 was used in Western Blot to identify critical enhancers of cardiac development and suggest that misregulation of these enhancers could contribute to cardiac defects in human patients.

Tue Apr 25 00:00:00 UTC 2023

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

PA5-29578 was used in Western Blot to suggest that paclitaxel modifies gene and protein expression of skin-related molecular markers, and impairs different physical, physiological, and biomechanical properties of the skin of cancer patients at a subclinical level.

Wed Feb 23 00:00:00 UTC 2022

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

Wed Feb 23 00:00:00 UTC 2022

Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis.

Biomedicines

Savin IA,Markov AV,Zenkova MA,Sen'kova AV

PA5-29578 was used in Immunohistochemistry to understand the molecular mechanisms of pulmonary fibrosis development.

Thu Apr 28 00:00:00 UTC 2022

Development of skeletal muscle atrophy and intermuscular adipose tissue in patients with early breast cancer treated with chemotherapy.

American journal of physiology. Cell physiology

Mallard J,Hucteau E,Bender L,Charlot A,Debrut L,Pflumio C,Trensz P,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

PA5-29578 was used in Western Blotting to demonstrate both IMAT development and muscle atrophy in patients with breast cancer who were treated with chemotherapy.

Sat Oct 01 00:00:00 UTC 2022

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

PA5-29569 was used in Western Blotting and Immunocytochemistry to test if MSC-derived exosomes could accelerate wound healing by enhancing the biological properties of the main cell types involved in the key phases of this process.

Wed Jun 09 00:00:00 UTC 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Wed Jun 09 00:00:00 UTC 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Wed Jun 09 00:00:00 UTC 2021

A step toward engineering thick tissues: Distributing microfibers within 3D printed frames.

Journal of biomedical materials research. Part A

Molde J,Steele JAM,Pastino AK,Mahat A,Murthy NS,Kohn J

PA5-29578 was used in Immunocytochemistry-Immunofluorescence to demonstrate that by combining FDM and airbrushing techniques we can engineer customizable hierarchical scaffolds for thick tissues that support increased cell growth and infiltration.

Sun Mar 01 00:00:00 UTC 2020

A step toward engineering thick tissues: Distributing microfibers within 3D printed frames.

Journal of biomedical materials research. Part A

Molde J,Steele JAM,Pastino AK,Mahat A,Murthy NS,Kohn J

Sun Mar 01 00:00:00 UTC 2020

1,25 Dihydroxyvitamin D3 Enhances the Antifibroid Effects of Ulipristal Acetate in Human Uterine Fibroids.

Reproductive sciences (Thousand Oaks, Calif.)

Ali M,Shahin SM,Sabri NA,Al-Hendy A,Yang Q

PA5-29578 was used in Western Blotting to suggest that combining vitamin D3 with ulipristal acetate produces additional and orchestrated anti-uterine fibroid effects.

Sat Jun 01 00:00:00 UTC 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA5-29578 was used in Western Blotting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 UTC 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

Thu Aug 15 00:00:00 UTC 2019

Diet-induced hepatic steatosis abrogates cell-surface LDLR by inducing de novo PCSK9 expression in mice.

The Journal of biological chemistry

Lebeau PF,Byun JH,Platko K,MacDonald ME,Poon SV,Faiyaz M,Seidah NG,Austin RC

PA5-29578 was used in Immunohistochemistry to examine the effect of hepatic steatosis on LDLR expression and circulating LDL cholesterol levels.

Fri Jun 07 00:00:00 UTC 2019

In vitro assessment of a novel, hypothermically stored amniotic membrane for use in a chronic wound environment.

International wound journal

McQuilling JP,Vines JB,Mowry KC

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Immunohistochemistry

Fri Dec 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor

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Gene Aliases: CIG; E330027I09; ED-B; FIBNEC; FINC; FN; Fn-1; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Human) Q585T2, (Mouse) P11276

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Entrez Gene ID: (Human) 2335, (Mouse) 14268, (Rat) 25661

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Function(s)

protease binding integrin binding protein binding collagen binding heparin binding peptidase activator activity identical protein binding mercury ion binding extracellular matrix structural constituent

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Fibronectin Polyclonal Antibody

Fibronectin Antibody (PA5-29578) in WB

Product Details

PA5-29578

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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