Invitrogen

Fibronectin Monoclonal Antibody (A17)

2 Published Figures
1 Reference
View all (99) Fibronectin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CSI 005-17-02

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Chicken, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A17

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074413

Product Specific Information

CSI 005-17-02 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). CSI 005-17-02 cross-reacts with human and chicken fibronectin. Other species have not been tested.

CSI 005-17-02 has been successfully used in Western blottin, ELISA, Immunohistochemistry and Immunoprecipitation procedures. The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.

The specificity of the CSI 005-17-02 epitope is located in the 120 kDa cell binding fragment.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; embryo-specific fibronectin 1 transcript; ferritin L subunit; ferritin L-chain; Fibronectin; fibronectin ED-A- region; fibronectin variable region; FN; Migration stimulating factor (MSF); migration-stimulating factor

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Gene Aliases: BOS_2065; CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Chicken) P11722, (Human) Q585T2

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Entrez Gene ID: (Bovine) 280794, (Chicken) 396133, (Human) 2335

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Function(s)
heparin binding peptidase activator activity protease binding integrin binding protein binding collagen binding identical protein binding mercury ion binding
Process(es)
angiogenesis acute-phase response cell-substrate junction assembly cell adhesion cell-matrix adhesion regulation of cell shape positive regulation of peptidase activity wound healing cell migration cell activation regulation of protein phosphorylation platelet degranulation response to ischemia calcium-independent cell-matrix adhesion positive regulation of cell proliferation glial cell migration response to wounding response to ozone positive regulation of gene expression peptide cross-linking extracellular matrix disassembly extracellular matrix organization integrin activation substrate adhesion-dependent cell spreading cellular response to vascular endothelial growth factor stimulus endodermal cell differentiation cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of axon extension positive regulation of fibroblast proliferation leukocyte migration positive regulation of chemotaxis response to glucocorticoid regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to mercury ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to prostaglandin E stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of transforming growth factor-beta secretion
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