Invitrogen

Fibronectin Monoclonal Antibody (FN-3), Alexa Fluor™ 488, eBioscience™

Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Fibronectin Antibody (53-9869-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human placenta using 10 µg/mL of Mouse IgG1 Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 10 µg/mL Anti-Human Fibronectin Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.

Fibronectin Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

53-9869-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FN-3

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11063704

Product Specific Information

Description: The FN-3 monoclonal antibody recognizes human fibronectin, a component of the extracellular matrix. This high molecular weight protein (almost 400 kDa) exists as an extracellular and a soluble plasma form. Fibronectin functions in cell adhesion and migration via integrins present on the interacting cell. Fibronectin also associates with collagen, actin and fibrins. In malignancies, fibronectin protein levels can be altered. In lung carninomas, expression is decreased. The FN-3 antibody recognizes a determinant on human cellular but not plasma fibronectin; this recognition is not lost upon trypsin treatment. Furthermore, FN-3 antibody has been shown to crossreact to bovine fibronectin.

Applications Tested: This FN-3 antibody has been tested by immunohistochemistry on FFPE human placenta (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955)) and can be used at less than or equal to 10 µg/mL. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds.

Biomedicines

Betriu N,Andreeva A,Alonso A,Semino CE

Published figure using Fibronectin monoclonal antibody (Product # 53-9869-82) in Immunocytochemistry

Fri Jul 29 00:00:00 UTC 2022

Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling.

British journal of pharmacology

Maamra M,Benayad OM,Matthews D,Kettleborough C,Atkinson J,Cain K,Bon H,Brand H,Parkinson M,Watson PF,Johnson TS

53-9869-82 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to develop a high affinity TG2-specific antibody against extracellular TG2 activity, with characteristics suitable for therapeutic development.

Wed Jun 01 00:00:00 UTC 2022

3D Extracellular Matrix Regulates the Activity of T Cells and Cancer Associated Fibroblasts in Breast Cancer.

Frontiers in oncology

Gao H,Tian Q,Zhu L,Feng J,Zhou Y,Yang J

Published figure using Fibronectin monoclonal antibody (Product # 53-9869-82) in Immunocytochemistry

Thu Apr 04 00:00:00 UTC 2024

Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 Patients.

SLAS discovery : advancing life sciences R & D

Marwick JA,Elliott RJR,Longden J,Makda A,Hirani N,Dhaliwal K,Dawson JC,Carragher NO

Published figure using Fibronectin monoclonal antibody (Product # 53-9869-82) in Immunocytochemistry

Fri Oct 01 00:00:00 UTC 2021

ILB^® resolves inflammatory scarring and promotes functional tissue repair.

NPJ Regenerative medicine

Hill LJ,Botfield HF,Begum G,Qureshi O,Vigneswara V,Masood I,Barnes NM,Bruce L,Logan A

53-9869-82 was used in Immunohistochemistry (PFA) to investigate the effects of a newly formulated low molecular weight dextran sulfate (LMW-DS), termed ILB®, to resolve inflammation and activate matrix remodelling in rodent and human disease models.

Thu Jan 07 00:00:00 UTC 2021

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

53-9869 was used in Immunocytochemistry-immunoflourescence to test the hypothesis that integrins are pro-fibrotic via regulation of functional interactions between tubular epithelial cells and renal fibroblasts.

Tue Sep 01 00:00:00 UTC 2020

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

Tue Sep 01 00:00:00 UTC 2020

A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts.

BMC biomedical engineering

Good RB,Eley JD,Gower E,Butt G,Blanchard AD,Fisher AJ,Nanthakumar CB

53-9869 was used in Immunocytochemistry to describe a robust, high content, pseudo-3D phenotypic assay to quantify mature fibrillar collagen deposition which is both physiologically relevant and amenable to high throughput compound screening.

Fri Mar 29 00:00:00 UTC 2024

A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts.

BMC biomedical engineering

Good RB,Eley JD,Gower E,Butt G,Blanchard AD,Fisher AJ,Nanthakumar CB

Fri Mar 29 00:00:00 UTC 2024

PEGylated pUR4/FUD peptide inhibitor of fibronectin fibrillogenesis decreases fibrosis in murine Unilateral Ureteral Obstruction model of kidney disease.

PloS one

Tomasini-Johansson BR,Zbyszynski PW,Toraason I,Peters DM,Kwon GS

53-9869 was used in Immunocytochemistry-immunoflourescence to study the ability of a PEGylated, high-affinity fibronectin-binding peptide to inhibit fibrosis in the Unilateral Ureteral Obstruction kidney disease model.

Fri Mar 22 00:00:00 UTC 2019

An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix.

Biology open

Qureshi OS,Bon H,Twomey B,Holdsworth G,Ford K,Bergin M,Huang L,Muzylak M,Healy LJ,Hurdowar V,Johnson TS

Sun Oct 15 00:00:00 UTC 2017

Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

Oncoimmunology

Jiang X,Seo YD,Chang JH,Coveler A,Nigjeh EN,Pan S,Jalikis F,Yeung RS,Crispe IN,Pillarisetty VG

53-9869 was used in Immunohistochemistry-immunofluorescence to demonstrate the potential of using tumour slice cultures to study the immune microenvironment of pancreatic ductal adenocarcinoma.

Sat Nov 04 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor

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Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Human) Q585T2

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Entrez Gene ID: (Human) 2335

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Function(s)

protease binding integrin binding protein binding collagen binding heparin binding peptidase activator activity identical protein binding mercury ion binding

Process(es)

angiogenesis cell activation regulation of protein phosphorylation platelet degranulation response to ischemia acute-phase response cell-substrate junction assembly cell adhesion calcium-independent cell-matrix adhesion positive regulation of cell proliferation glial cell migration regulation of cell shape response to wounding response to ozone positive regulation of gene expression positive regulation of peptidase activity peptide cross-linking extracellular matrix disassembly extracellular matrix organization integrin activation substrate adhesion-dependent cell spreading cellular response to vascular endothelial growth factor stimulus endodermal cell differentiation cellular response to platelet-derived growth factor stimulus wound healing negative regulation of apoptotic process positive regulation of axon extension positive regulation of fibroblast proliferation leukocyte migration positive regulation of chemotaxis response to glucocorticoid regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to mercury ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to prostaglandin E stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of transforming growth factor-beta secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Fibronectin Monoclonal Antibody (FN-3), Alexa Fluor™ 488, eBioscience™

Fibronectin Antibody (53-9869-82) in IHC (P)

Product Details

53-9869-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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