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Invitrogen
Filamin B Monoclonal Antibody (11E2D2)
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FIGURE: 1 / 6
Filamin B Antibody (MA5-49226) in ICC/IF
Immunocytochemistry analysis of Filamin B in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Filamin B Monoclonal antibody (Product # MA5-49226) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incuba... View More
Product Details
MA5-49226
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
11E2D2
Immunogen
E.coli-derived human Filamin B/FLNB recombinant protein (Position: Q397-D701).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074884
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell. IHC: human lung cancer tissue, human lymph node of rectal cancer tissue, human metaplasia of squamous cells of the renal pelvis tissue, human spleen tissue. ICC/IF: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
FOXK1 is expressed in these cells and regulates cell cycle progression through an interaction with its downstream target the cyclin-dependent kinase inhibitor p21 (CIP). Loss of FOXK1 in mice results in growth retardation and a severe impairment in skeletal muscle regeneration following injury. FOXK1 also shows expression in immature tissues of brain, eye, heart, lung and thymus. It also is predominantly expressed in many malignant tissues, such as tumors of the brain, colon and lymph node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABP-278; ABP-280 homolog; actin binding protein 278; Actin-binding-like protein; Beta-filamin; Fh1; filamin B, beta; Filamin homolog 1; Filamin-3; Filamin-B; FilaminB; FLN-B; Larsen syndrome 1 (autosomal dominant); Thyroid autoantigen; Truncated ABP; Truncated actin-binding protein
Gene Aliases: ABP-278; ABP-280; AOI; FH1; FLN-B; FLN1L; FLN3; FLNB; LRS1; SCT; TABP; TAP
UniProt ID: (Human) O75369
Entrez Gene ID: (Human) 2317
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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