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Invitrogen
Flotillin 1 Polyclonal Antibody
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FIGURE: 1 / 4
Flotillin 1 Antibody (PA5-19713) in WB
Western blot was performed using Anti- Flotillin 1 Polyclonal Antibody (Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. An uncharacterized band was observed at ~32KDa across all the samples and ~60KDa in THP-1 sample alone. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts... View More
Product Details
PA5-19713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Flotillin 1.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981198
Product Specific Information
For Western Blot, this antibody has non-specific bands at 35 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with many species based on sequence homology.
Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.
Target Information
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DADB-118P11.2; Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855
Gene Aliases: FLOT1; reggie-2
UniProt ID: (Human) O75955, (Mouse) O08917
Entrez Gene ID: (Human) 10211, (Mouse) 14251
membrane raft assembly
positive regulation of cytokine production
positive regulation of protein phosphorylation
regulation of receptor internalization
endocytosis
axonogenesis
axon guidance
extracellular matrix disassembly
positive regulation of protein binding
positive regulation of interferon-beta production
dsRNA transport
positive regulation of heterotypic cell-cell adhesion
positive regulation of toll-like receptor 3 signaling pathway
response to endoplasmic reticulum stress
regulation of Rho protein signal transduction
plasma membrane raft assembly
positive regulation of endocytosis
positive regulation of skeletal muscle tissue development
protein homooligomerization
positive regulation of cell adhesion molecule production
protein kinase C signaling
cellular response to exogenous dsRNA
protein localization to plasma membrane
establishment of protein localization to plasma membrane
positive regulation of myoblast fusion
positive regulation of cell junction assembly
protein localization to membrane raft
positive regulation of cell-cell adhesion mediated by cadherin
positive regulation of synaptic transmission, dopaminergic
regulation of toll-like receptor 4 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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