Bioss

Fx1A Polyclonal Antibody

Product Details

BS-1338R

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fx1A protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Binding of anti-Fx1A to Heymann nephritis antigens (HA) on rat glomerular epithelial cells (GECs) in culture leads to capping and disappearance of antigens from the cell surface. This process may contribute to the formation of glomerular subepithelial immune deposits in vivo. The authors differentially extracted GECs to determine whether HA redistribution is mediated by cytoskeletal components. Observations were made by phase-contrast and immunofluorescence microscopy on primary and passaged GECs in monolayer culture and by spectrofluorimetry on GECs in suspension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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