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G6B Antibody (PA5-87682) in WB
Western blot analysis of G6B in extracts of various cell lines. Samples were incubated with G6B Polyclonal antibody (Product # PA5-87682) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-87682
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-142 of human C6orf25 (NP_6121211)
Conjugate
Form
Liquid
Concentration
2.25 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804343
Product Specific Information
Immunogen sequence: NPGASLDGRP GDRVNLSCGG VSHPIRWVWA PSFPACKGLS KGRRPILWAS SSGTPTVPPL QPFVGRLRSL DSGIRRLELL LSAGDSGTFF CKGRHEDESR TVLHVLGDRT YCKAPGPTHG SVYPQ; Positive Samples: K-562, Jurkat, SW620, Mouse intestine, Mouse pancreas, Rat pancreas; Cellular Location: Cell membrane, Endoplasmic reticulum, Golgi apparatus, Golgi apparatus, Single-pass type I membrane protein
Target Information
G6B is a member of the immunoglobulin (Ig) superfamily with 241 amino acids and is a glycosylated, plasma membrane-bound cell surface receptor, but soluble isoforms encoded by some transcript variants have been found in the endoplasmic reticulum and Golgi before being secreted. Multiple transcript variants encoding different isoforms have been reported. RT-PCR, Western blot, and flow cytometric analyses suggest that G6B is usually expressed on platelets and cross linking of G6B inhibits platelet aggregation and activation in a calcium-independent manner by agonists such as ADP and collagen-related peptide. G6B is an inhibitory surface receptor on platelets and may be an antithrombotic drug target. Isoform B is a putative inhibitory receptor. Isoform A may be its activating counterpart. It is known to bind to heparin. G6B is usually expressed in a restricted set of hematopoietic cell lines like K562, Molt4 and Jurkat and not detected in the cell lines like U937, Raji, Tk, HeLa, NKL, NK62 and YT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DADB-110M10.2; immunoglobulin receptor; Immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing platelet receptor; ITIM-receptor G6b-B; Megakaryocyte and platelet inhibitory receptor G6b; Protein G6b
Gene Aliases: AU023871; C6orf25; G6B; G6B-B; MPIG6B; NG31
UniProt ID: (Human) O95866, (Mouse) D7PDD4
Entrez Gene ID: (Human) 80739, (Mouse) 106722, (Rat) 406865
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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