Invitrogen

G6PD Monoclonal Antibody (5E12)

G6PD Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

G6PD Antibody (MA5-15918) in ICC/IF

Immunofluorescence analysis of G6PD was performed using A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with G6PD Monoclonal Antibody (5E12) (Product # MA5-15918) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1... View More

G6PD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

G6PD Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with G6PD siRNA and reduction of signal was observed in Western Blot using G6PD Monoclonal Antibody (Product # MA5-15918). {KD}

Product Details

MA5-15918

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5E12

Immunogen

Purified recombinant fragment of human G6PD expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11155569

Product Specific Information

MA5-15918 targets G6PD in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15918 immunogen is purified recombinant fragment of human G6PD expressed in E. Coli. .

MA5-15918 detects G6PD which has a predicted molecular weight of approximately 59kDa.

Target Information

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Placental growth factor regulates the pentose phosphate pathway and antioxidant defense systems in human retinal endothelial cells.

Journal of proteomics

Saddala MS,Lennikov A,Huang H

MA5-15918 was used in Western Blotting to provide new insights into protein expression patterns and enables the identification of many attractive candidates for investigation of PPP pathway role in the activation of the antioxidant defense system in DR.

Wed Apr 15 00:00:00 UTC 2020

Placental growth factor negatively regulates retinal endothelial cell barrier function through suppression of glucose-6-phosphate dehydrogenase and antioxidant defense systems.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Huang H,Lennikov A,Saddala MS,Gozal D,Grab DJ,Khalyfa A,Fan L

MA5-15918 was used in Western Blot to report that placental growth factor (PlGF) negatively affects the endothelial cell (EC) barrier function through a novel regulatory mechanism.

Sun Dec 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: G6PD; Glucose-6-phosphate 1-dehydrogenase

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Gene Aliases: G6PD; G6PD1

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UniProt ID: (Human) P11413

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Entrez Gene ID: (Human) 2539

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Function(s)

glucose-6-phosphate dehydrogenase activity protein binding glucose binding identical protein binding protein homodimerization activity NADP binding

Process(es)

glucose metabolic process pentose-phosphate shunt lipid metabolic process cholesterol biosynthetic process NADP metabolic process NADPH regeneration glutathione metabolic process pentose-phosphate shunt, oxidative branch negative regulation of protein glutathionylation response to organic cyclic compound pentose biosynthetic process substantia nigra development response to food cellular response to oxidative stress erythrocyte maturation regulation of neuron apoptotic process response to ethanol ribose phosphate biosynthetic process glucose 6-phosphate metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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G6PD Monoclonal Antibody (5E12)

G6PD Antibody (MA5-15918) in ICC/IF

Product Details

MA5-15918

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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