Proteintech

GA repeat Polyclonal Antibody

14 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

GA repeat Antibody (24492-1-AP) in ELISA

GA repeat Antibody (Product # 24492-1-AP) tested in ELISA. GA repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of GA repeat antibody (Product # 24492-1-AP) . Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Product Image Gallery

Product Details

24492-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-Dependent

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is used to detect the "GA repeat" sequence. This antibody detects the GAGAGAGAGA peptide with dilution 1:32,000 in ELISA.

Target Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PIKFYVE inhibition mitigates disease in models of diverse forms of ALS.

Cell

Hung ST,Linares GR,Chang WH,Eoh Y,Krishnan G,Mendonca S,Hong S,Shi Y,Santana M,Kueth C,Macklin-Isquierdo S,Perry S,Duhaime S,Maios C,Chang J,Perez J,Couto A,Lai J,Li Y,Alworth SV,Hendricks E,Wang Y,Zlokovic BV,Dickman DK,Parker JA,Zarnescu DC,Gao FB,Ichida JK

Thu Feb 16 00:00:00 UTC 2023

Reversing lysosome-ribosome circuit dysregulation mitigates C9FTD/ALS neurodegeneration and behaviors.

Human molecular genetics

Ma L,Liang C,Wang J,Chang Q,Wang Y,Zhang W,Du Y,Sadan J,Chen JF

Thu Apr 06 00:00:00 UTC 2023

C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons.

Life science alliance

Marchi PM,Marrone L,Brasseur L,Coens A,Webster CP,Bousset L,Destro M,Smith EF,Walther CG,Alfred V,Marroccella R,Graves EJ,Robinson D,Shaw AC,Wan LM,Grierson AJ,Ebbens SJ,De Vos KJ,Hautbergue GM,Ferraiuolo L,Melki R,Azzouz M

Thu Sep 01 00:00:00 UTC 2022

Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.

Nature neuroscience

Szebényi K,Wenger LMD,Sun Y,Dunn AWE,Limegrover CA,Gibbons GM,Conci E,Paulsen O,Mierau SB,Balmus G,Lakatos A

Mon Nov 01 00:00:00 UTC 2021

SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.

Journal of cell science

Malnar M,Rogelj B

Fri Feb 19 00:00:00 UTC 2021

Active poly-GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model.

EMBO molecular medicine

Zhou Q,Mareljic N,Michaelsen M,Parhizkar S,Heindl S,Nuscher B,Farny D,Czuppa M,Schludi C,Graf A,Krebs S,Blum H,Feederle R,Roth S,Haass C,Arzberger T,Liesz A,Edbauer D

Fri Feb 07 00:00:00 UTC 2020

Altered MICOS Morphology and Mitochondrial Ion Homeostasis Contribute to Poly(GR) Toxicity Associated with C9-ALS/FTD.

Cell reports

Li S,Wu Z,Li Y,Tantray I,De Stefani D,Mattarei A,Krishnan G,Gao FB,Vogel H,Lu B

Tue Aug 04 00:00:00 UTC 2020

Smcr8 deficiency disrupts axonal transport-dependent lysosomal function and promotes axonal swellings and gain of toxicity in C9ALS/FTD mouse models.

Human molecular genetics

Liang C,Shao Q,Zhang W,Yang M,Chang Q,Chen R,Chen JF

Sun Dec 01 00:00:00 UTC 2019

Neurodegeneration in frontotemporal lobar degeneration and motor neurone disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.

Neuropathology and applied neurobiology

Davidson Y,Robinson AC,Liu X,Wu D,Troakes C,Rollinson S,Masuda-Suzukake M,Suzuki G,Nonaka T,Shi J,Tian J,Hamdalla H,Ealing J,Richardson A,Jones M,Pickering-Brown S,Snowden JS,Hasegawa M,Mann DM

Fri Apr 01 00:00:00 UTC 2016

Neuropathology and applied neurobiology

Davidson Y,Robinson AC,Liu X,Wu D,Troakes C,Rollinson S,Masuda-Suzukake M,Suzuki G,Nonaka T,Shi J,Tian J,Hamdalla H,Ealing J,Richardson A,Jones M,Pickering-Brown S,Snowden JS,Hasegawa M,Mann DM

Fri Apr 01 00:00:00 UTC 2016

Neuropathology and applied neurobiology

Davidson Y,Robinson AC,Liu X,Wu D,Troakes C,Rollinson S,Masuda-Suzukake M,Suzuki G,Nonaka T,Shi J,Tian J,Hamdalla H,Ealing J,Richardson A,Jones M,Pickering-Brown S,Snowden JS,Hasegawa M,Mann DM

Fri Apr 01 00:00:00 UTC 2016

Neuropathology and applied neurobiology

Davidson Y,Robinson AC,Liu X,Wu D,Troakes C,Rollinson S,Masuda-Suzukake M,Suzuki G,Nonaka T,Shi J,Tian J,Hamdalla H,Ealing J,Richardson A,Jones M,Pickering-Brown S,Snowden JS,Hasegawa M,Mann DM

Fri Apr 01 00:00:00 UTC 2016

C9orf72 Hexanucleotide Expansions Are Associated with Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.

Stem cells (Dayton, Ohio)

Dafinca R,Scaber J,Ababneh N,Lalic T,Weir G,Christian H,Vowles J,Douglas AG,Fletcher-Jones A,Browne C,Nakanishi M,Turner MR,Wade-Martins R,Cowley SA,Talbot K

Mon Aug 01 00:00:00 UTC 2016

Stem cells (Dayton, Ohio)

Dafinca R,Scaber J,Ababneh N,Lalic T,Weir G,Christian H,Vowles J,Douglas AG,Fletcher-Jones A,Browne C,Nakanishi M,Turner MR,Wade-Martins R,Cowley SA,Talbot K

Mon Aug 01 00:00:00 UTC 2016

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 UTC 2016

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 UTC 2016

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 UTC 2016

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 UTC 2016

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GA repeat Polyclonal Antibody

GA repeat Antibody (24492-1-AP) in ELISA

Product Details

24492-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Dot blot (DB)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Performance Guarantee

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