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Alomone Labs, LTD
GABA(A) alpha 1 Receptor (extracellular) Polyclonal Antibody
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FIGURE: 1 / 5
GABA(A) alpha 1 Receptor (extracellular) Antibody (AGA-001-200UL) in ICC/IF
Expression of GABRA1 in rat PC12 cells - Cell surface detection of GABRA1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-GABA (A) alpha 1 Receptor (extracellular) Antibody (#AGA-001), (1:100) followed by goat Anti-rabbit-AlexaFluor-594secondary Antibody . B. Merge image of A and live view of the cells.
Product Details
AGA-001-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABAA alpha 1; GABAAR alpha1; GABAAR subunit alpha-1; GABRA 1; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid A receptor, alpha 1; Gamma-aminobutyric acid receptor subunit alpha-1
Gene Aliases: ECA4; EIEE19; EJM; EJM5; Gabra-1; GABRA1
UniProt ID: (Human) P14867, (Rat) P62813, (Mouse) P62812
Entrez Gene ID: (Human) 2554, (Rat) 29705, (Mouse) 14394
GABA-A receptor activity
drug binding
GABA receptor activity
GABA-gated chloride ion channel activity
inhibitory extracellular ligand-gated ion channel activity
chloride channel activity
protein binding
benzodiazepine receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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