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Invitrogen
GABRA1 Polyclonal Antibody
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GABRA1 Antibody (PA1-4658) in WB
Western blot was performed using Anti-GABRA1 Antibody (Product # PA1-4658) and a 51 kDa band corresponding to GABRA1 was observed in only brain specific tissues while other tissues are reported to be low to negative for its expression. Tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3), Mouse Lung (Lane 4), Rat Lung (Lane 5), Mouse Heart (Lane 6) and Rat Heart (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferr... View More
Product Details
PA1-4658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein from the cytoplasmic loop of the alpha 1 subunit of rat GABRA1
Conjugate
Form
Liquid
Concentration
~0.15 mg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_559218
Product Specific Information
This antibody is predicted to react with bovine, canine, human and non-human primate based on 100% sequence homology.
This antibody is specific for the ~51 kDa alpha1-subunit of the GABAA Receptor in Western blots of rat brain.
Target Information
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GABA(A) receptor subunit alpha-1; GABAA alpha 1; GABAAR alpha1; GABAAR subunit alpha-1; GABRA 1; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid A receptor, alpha 1; Gamma-aminobutyric acid receptor subunit alpha-1
Gene Aliases: Gabra-1; Gabra1
UniProt ID: (Rat) P62813, (Mouse) P62812
Entrez Gene ID: (Rat) 29705, (Mouse) 14394
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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